This is only clones without inserts ('empty vectors'). Many other vectors are represented in the repository (try 'search by reference sequence' or 'view collection').

Explanation of Terms

  Clone ID Vector Name Description Cloning Strategy Tag Special MTA Use Restriction  
1 EvNO00401221 pVALIUM1 Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
2 EvNO00422221 pVALIUM10 Drosophila expression vector for high expression of hairpin insert. loxP, and attB recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with ftz intron and gypsy insulator. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
3 EvNO00422222 pWALIUM10 Drosophila expression vector for high expression of hairpin insert. Hsp70 promoter with 5xUAS, two gypsy insulators, and two ftz introns. AmpR in bacteria; white marker in drosophila. loxP and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
4 EvNO00422223 pVALIUM20 Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with ftz intron, two gypsy insulators, 5xUAS and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
5 EvNO00422224 pVALIUM22 Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
6 EvNO00422225 pVALIUM10-roe Drosophila expression vector for Recombination cloning, Over Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, SV40 poly A, vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
7 EvNO00422226 pVALIUM10-moe Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
8 EvNO00422227 pWALIUM10-roe Drosophila expression vector for Recombination cloning, Over-Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two Gypsy insulators, two 5xUAS, Hsp70 promoter, with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA Academic and non-profit labs
9 EvNO00422228 pWALIUM10-moe Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA Academic and non-profit labs
10 EvNO00422230 pWALIUM20 Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
11 EvNO00422231 pWALIUM22 Drosophila expression vector. loxP, and GATEWAY recombination sites with MCS. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, p-transposase promoter with ftz intron and K10 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs