This is only clones without inserts ('empty vectors'). Many other vectors are represented in the repository (try 'search by reference sequence' or 'view collection').

Explanation of Terms

  Clone ID Vector Name Description Cloning Strategy Tag Special MTA Use Restriction  
1 EvNO00013281 pKT176 Yeast fluorescent (tdimer2) vector with PTEF promoter; amp resistance (CaURA3 for yeast); restriction enzyme cloning. multiple cloning site TSIEN Academic and non-profit labs
2 EvNO00013282 pKT178 Yeast fluorescent (tdimer2) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning. multiple cloning site TSIEN Academic and non-profit labs
3 EvNO00013283 pKT146 Yeast fluorescent (tdimer2) vector; amp resistance (HIS5 for yeast); restriction enzyme cloning. multiple cloning site TSIEN Academic and non-profit labs
4 EvNO00013284 pHR-MMPCreGFP Self-excising mammalian retroviral vector with Cre-GFP enzyme-marker and LoxP sites; ampicillin resistance loxP Academic and non-profit labs
5 EvNO00013285 pEpuro Eukaryotic expression vector (unknown promoter); amp resistance (puromycin for mammalian selection); restriction enzyme cloning. Academic and non-profit labs
6 EvNO00017254 pPRIME-TET-GFP-recipient Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, fluorescent (GFP) marker; amp resistance; MAGIC bacterial mating cloning. MAGIC mating system Academic and non-profit labs
7 EvNO00017257 pPRIME-CMV-dsRed-recipient Lentiviral expression vector for shRNA expression with fluorescent (DsRed) marker; amp resistance; MAGIC bacterial mating cloning. MAGIC mating system Academic and non-profit labs
8 EvNO00017259 pPRIME-CMV-LNGFR-recipient Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning. MAGIC mating system Academic and non-profit labs
9 EvNO00017260 pPRIME-TREX-GFP-recipient Lentiviral expression vector for shRNA expression with inducible (TREX) promoter, fluorescent (GFP marker); amp resistance; MAGIC bacterial mating cloning. MAGIC mating system Academic and non-profit labs
10 EvNO00023114 pJP1520 Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (puromycin for mammalian selection); recombinational cloning. loxP; Creator&#153 or compatible No restriction
11 EvNO00023115 pJP1563 Retroviral expression vector with CMV promoter, MMV-type 5p and 3p LTRs; amp resistance (blasticidin for mammalian selection); recombinational cloning. loxP; Creator&#153 or compatible No restriction
12 EvNO00025938 pEF1 Cpo Myc His Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal Myc then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning. multiple cloning site; CpoI enzyme-based directional cloning Myc; His tag Academic and non-profit labs
13 EvNO00025939 pEF1 Cpo V5 His Mammalian and in vitro expression vector with EF1-alpha promoter and T7, adds C-terminal V5 epitope then His tag; amp resistance (neomycin for mammalian selection); CpoI-based in-frame, single-cut directional cloning. multiple cloning site; CpoI enzyme-based directional cloning V5 tag; His tag Academic and non-profit labs
14 EvNO00025949 pFA6a-mRFP1-kanMX6 Yeast fluorescent (RFP) vector with PTEF promoter; amp resistance (kanamycin for yeast); restriction enzyme cloning. multiple cloning site TSIEN Academic and non-profit labs
15 EvNO00025959 pIRES2 lac smGFP Mammalian expression vector with fluorescent tag (soluble modified GFP, smGFP), CMV promoter, lac promoter; kanamycin resistance (neomycin for mammalian selection); restriction enzyme cloning. smGFP Academic and non-profit labs
16 EvNO00025962 pSP72 syn-CTAP Cloning and in vitro transcription vector with synthetic CTAP ORF added; amp resistance; restriction enzyme cloning. multiple cloning site syn-CTAP Academic and non-profit labs
17 EvNO00025963 pSP72 syn-CTAP AphAI Vector with kanR gene inside CTAP tag ORF (synthetic CTAP designed to avoid repeats, optimize for human codon usage); useful for two-step Red recombination BAC mutagenesis. multiple cloning site; Red recombination IgG site 1; IgG site 2; IgG site 1; IgG site 2; Protein C Academic and non-profit labs
18 EvNO00025964 pJK-NTAP Cloning and in vitro transcription vector with synthetic NTAP ORF added (synthetic NTAP designed to avoid repeats, optimize for human codon usage); amp resistance; restriction enzyme cloning. multiple cloning site PTP NTAP; IgG 1; IgG 2; Protein C epitope Academic and non-profit labs
19 EvNO00025965 pNTAP-TSR Vector with kanR gene insert NTAP tag ORF; useful for two-step Red recombination BAC mutagenesis approach. multiple cloning site; Red recombination Academic and non-profit labs
20 EvNO00025966 pST76K-SR Shuttle vector for BAC mutagenesis (for example of infectious viral genome into BAC), ts for DNA replication; kanamycin resistance; in vivo selection for cointegrants followed by resolution to clone in BACs multiple cloning site Academic and non-profit labs
21 EvNO00061595 pAAV-GFP Adeno-associated viral (AAV) vector, CMV promoter drives GFP fluorescent marker; amp resistance; restriction enzyme cloning. Academic and non-profit labs
22 EvNO00061596 pAAV-MCS Adeno-associated viral (AAV) vector with CMV promoter, beta globin intron and polyA signal, maximum capacity for insertion 2.3 kb; amp resistance; restriction enzyme cloning. Academic and non-profit labs
23 EvNO00061598 pAAV-MCS6-IZsGreen Adeno-associated viral (AAV) vector with CMV promoter, SV40 polyA signal, maximum capacity for insertion 2.3 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
24 EvNO00061600 pAAV-MCS8-IZsGreen Adeno-associated viral (AAV) vector with CMV promoter, human beta globin intron, SV40 polyA signal, maximum capacity for insertion 1.7 kb, has IRES-ZsGreen (IRES-fluorescent tag) as marker of transduced cells; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
25 EvNO00061604 pHAGE-CMV-fullEF1a-IRES-ZsGreen Lentiviral vector with internal EF1a promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
26 EvNO00061605 pHAGE-CMV-MCS-IRES-ZsGreen Lentiviral vector with internal CMV promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
27 EvNO00061606 pHDM-G Helper plasmid for murine leukemia virus (MLV) or lentiviral vectors, CMV promoter drives VSV-G envelope protein, contains human beta globin intron and polyA; amp resistance; not a cloning vector. Academic and non-profit labs
28 EvNO00061607 pHDM-Hgpm2 Helper plasmid for lentiviral vector, has codon optimized HIV gag-pol driven by CMV promoter; amp resistance; not a cloning vector. Academic and non-profit labs
29 EvNO00061608 pHDM-tat1b Helper plasmid for lentiviral vector, HIV tat driven by CMV promoter; amp resistance; not a cloning vector. Academic and non-profit labs
30 EvNO00061610 pHRST-eGFP Lentiviral vector with internal CMV promoter that drives eGFP fluorescent tag; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
31 EvNO00061612 pHRST-IRES-eGFP Lentiviral vector with internal CMV promoter, has IRES-eGFP (IRES-fluorescent tag) to mark transduced cells; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
32 EvNO00061613 pMD-MLVogp Helper plasmid for murine leukemia virus (MLV) retroviral vectors, encodes MLV gag-pol polyproteins, has human beta-globin intron and polyA signal; amp resistance; not a cloning vector. Academic and non-profit labs
33 EvNO00061616 pRC/CMV-rev1b Helper plasmid for lentiviral vector, rev1b driven by CMV promoter; amp resistance; not a cloning vector. Academic and non-profit labs
34 EvNO00061622 pEnt3 Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning. Academic and non-profit labs
35 EvNO00061624 pGP704 Vector that accepts insert for gene targeting approach in bacteria; amp, streptomycin (tetracycline? See map); restriction enzyme cloning. Academic and non-profit labs
36 EvNO00061625 pIVET1 Vector for bacterial in vivo expression technology (IVET) transposon insertion-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. Academic and non-profit labs
37 EvNO00061626 pIVET8 Vector for bacterial in vivo expression technology (IVET) transposon insert-based approach to identification of promoters that drive expression of promotorless marker in the vector; amp resistance; restriction enzyme cloning. Academic and non-profit labs
38 EvNO00061628 pMEnt Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning. Academic and non-profit labs
39 EvNO00061629 pMycoMar Vector for transposon-mediated mutagenesis in mycobacteria, with ts origin of replication, mariner transposase and transposon, mycobacterial promoter for selectable marker; gentamycin, kanamycin, tetracycline resistance (kanamycin for selection of insertions in mycobacteria); restriction enzyme cloning. Academic and non-profit labs
40 EvNO00061634 pHAGE-CMV-eGFP-W Lentiviral vector that expresses eGFP fluorescent marker from CMV promoter, with WPRE; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
41 EvNO00061635 pHAGE-PGK-MCS-IRES-ZsGreen-W Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
42 EvNO00061636 pHAGE-shortEF1a-MCS-IRES-ZsGreen-W Lentiviral vector that expresses insert from internal short EF1a promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
43 EvNO00061637 pHAGE-UBC-MCS-IRES-ZsGreen-W Lentiviral vector that expresses insert from internal UBC promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; amp resistance; restriction enzyme cloning. multiple cloning site Academic and non-profit labs
44 EvNO00061638 pHRST-nCre Lentiviral vector that expresses nuclear localization signal (nls)-containing Cre recombinase from internal CMV promoter; amp resistance; restriction enzyme cloning. Academic and non-profit labs
45 EvNO00061657 pNJ17 Vector for transposon-based mutagenesis in bacteria, with arabinose-inducible promoter to drive expression of gene adjacent to transposon insert (modified promoter-out approach); kanamycin resistance; restriction enzyme cloning. Academic and non-profit labs
46 EvNO00079526 pLP-EGFP-C1 Empty vector that adds EGFP fusion to insert (discontinued by supplier); kan resistance; Creator (Cre/Lox-type) recombinational cloning. Creator&#153 or compatible Academic and non-profit labs
47 EvNO00079527 pLPS-3'EGFP Empty vector that adds EGFP fusion to insert (discontinued by supplier); kan resistance; Creator (Cre/Lox-type) recombinational cloning. Creator&#153 or compatible; recombinational cloning EGFP Academic and non-profit labs
48 EvNO00285801 pBY011 Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning. Gateway® or compatible Academic and non-profit labs
49 EvNO00292861 pCW62 Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning. His; Flag No restriction
50 EvNO00401201 pWE-p2A-mRFP Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. Academic and non-profit labs
51 EvNO00401203 pCW63 Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. Academic and non-profit labs
52 EvNO00401205 pWE3-F2 Mammalian expression vector for lentiviral work. Uses PHAGE recombinational cloning (compatible with GATEWAY). HA-FLAG tag added to frame 2 fusion format inserts during expression. Viral components on backbone improve expression in mammalian cell lines. Academic and non-profit labs
53 EvNO00401221 pVALIUM1 Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
54 EvNO00422221 pVALIUM10 Drosophila expression vector for high expression of hairpin insert. loxP, and attB recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with ftz intron and gypsy insulator. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
55 EvNO00422222 pWALIUM10 Drosophila expression vector for high expression of hairpin insert. Hsp70 promoter with 5xUAS, two gypsy insulators, and two ftz introns. AmpR in bacteria; white marker in drosophila. loxP and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
56 EvNO00422223 pVALIUM20 Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Hsp70 promoter with ftz intron, two gypsy insulators, 5xUAS and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
57 EvNO00422224 pVALIUM22 Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
58 EvNO00422225 pVALIUM10-roe Drosophila expression vector for Recombination cloning, Over Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, SV40 poly A, vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
59 EvNO00422226 pVALIUM10-moe Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
60 EvNO00422227 pWALIUM10-roe Drosophila expression vector for Recombination cloning, Over-Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrni.org/TRiP-HOME.html Two Gypsy insulators, two 5xUAS, Hsp70 promoter, with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA Academic and non-profit labs
61 EvNO00422228 pWALIUM10-moe Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP, and MCS. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, and SV40 polyA. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA Academic and non-profit labs
62 EvNO00422230 pWALIUM20 Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
63 EvNO00422231 pWALIUM22 Drosophila expression vector. loxP, and GATEWAY recombination sites with MCS. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, p-transposase promoter with ftz intron and K10 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. Academic and non-profit labs
64 EvNO00432212 pWE-p2A-mRFP Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. Academic and non-profit labs
65 EvNO00438082 pHDMG Envelope plasmid used for lentivirus production. Mammalian expression of VSV-G driven by CMV promoter. Ampicillin resistance in bacteria. Academic and non-profit labs
66 EvNO00438083 pMDL Packaging plasmid used for viral production. Mammalian expression of Gag-Pol driven by CMV promoter. Ampicillin resistance in bacteria. Map inferred from end read sequence and anecdotal information. Reference sequence is END READ ONLY. Academic and non-profit labs
67 EvNO00438084 VSV-G Envelope plasmid used for lentivirus production. Mammalian expression of VSV-G driven by CMV promoter. Ampicillin resistance in bacteria. Map inferred from end read sequence and anecdotal information. Reference sequence is END READ ONLY. Academic and non-profit labs
68 EvNO00438085 pLKO-scramble Control hairpin designed to demonstrate positive infection via conferred puromycin resistance, but will not target a protein in human genome. Academic and non-profit labs
69 EvNO00438621 pCW45 Lentiviral cDNA expression vector with ampicillin resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning. Academic and non-profit labs
70 EvNO00483428 pQUAS-WALIUM20 Empty Drosophila expression vector Academic and non-profit labs