Vector: pQUAS-WALIUM20
Number of plasmids with this backbone: | 1 |
Name: | pQUAS-WALIUM20 |
Description: | Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, 94-bp QUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 10372 |
Properties: | drosophila in vitro and in vivo expression |
Vector Map: | pQUAS-WALIUM20 Map-corrected.pdf |
Vector Sequence: | pQUAS-WALIUM20.txt |
Comments: | Two copies of loxp-5xUAS sequences of "pWALIUM20" are deleted and replaced with the 94-bp QUAS sequence (five copies of the QF binding site) |
Type | Name | Description | Start Position | End Position |
promoter | Hsp70 | 5822 | 6081 |
Authors:
Author Name | Author Type | Creation Date |
Norbert Perrimon | Academic researcher | |
Rong Tao | Vector creator |
Publications:
PMID | Title |
26320097 | The Transgenic RNAi Project at Harvard Medical School: Resources and Validation |
Parent Vector:
Name | Comments |