Vector: pQUAS-WALIUM20

Number of plasmids with this backbone: 1
Description: Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: Two gypsy insulators, 94-bp QUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 10372
Properties: drosophila in vitro and in vivo expression
Vector Map: pQUAS-WALIUM20 Map-corrected.pdf
Vector Sequence: pQUAS-WALIUM20.txt
Comments: Two copies of loxp-5xUAS sequences of "pWALIUM20" are deleted and replaced with the 94-bp QUAS sequence (five copies of the QF binding site)


Type Name Description Start Position End Position
promoter Hsp70 5822 6081


Author Name Author Type Creation Date
Norbert Perrimon Academic researcher
Rong Tao Vector creator


PMID Title
26320097 The Transgenic RNAi Project at Harvard Medical School: Resources and Validation

Parent Vector:

Name Comments