Vector: pQUAS-WALIUM20

Number of plasmids with this backbone: 1
Name: pQUAS-WALIUM20
Description: Drosophila expression vector. loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, 94-bp QUAS, Hsp70 promoter with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 10372
Properties: drosophila in vitro and in vivo expression
Vector Map: pQUAS-WALIUM20 Map-corrected.pdf
Vector Sequence: pQUAS-WALIUM20.txt
Comments: Two copies of loxp-5xUAS sequences of "pWALIUM20" are deleted and replaced with the 94-bp QUAS sequence (five copies of the QF binding site)

Features:

Type Name Description Start Position End Position
promoter Hsp70 5822 6081

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher
Rong Tao Vector creator

Publications:

PMID Title
26320097 The Transgenic RNAi Project at Harvard Medical School: Resources and Validation

Parent Vector:

Name Comments