Vector: pLKO-scramble
Number of plasmids with this backbone: | 1 |
Name: | pLKO-scramble |
Description: | Control hairpin designed to demonstrate positive infection via conferred puromycin resistance, but will not target a protein in human genome. |
Synonyms: | SCR, scramble, scrambled shRNA |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 7095 |
Properties: | viral production |
Vector Map: | pLKO.1-puro.jpg |
Vector Sequence: | pLKO-scramble.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
primer site | hU6 | 1 | 19 | |
hairpin | scrambled shRNA | CCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGG | 153 | 200 |
polypurine tract | cppt | 251 | 266 | |
mammalian antibiotic | puroR | 945 | 1544 | |
polypurine tract | cPPT #2 | 1655 | 1670 | |
viral LTR | 3' LTR | 1725 | 1905 | |
primer site | T7 | 2293 | 2313 | |
primer site | M13 forward | 2322 | 2339 | |
bacterial selection antibiotic | AmpR | 3264 | 3923 | |
primer site | M13 reverse | 5071 | 5091 | |
primer site | T3 | 5109 | 5128 | |
viral LTR | 5' | 5381 | 5561 | |
viral packaging component | psi | 5672 | 5716 | |
viral response element | RRE | 6226 | 6459 |
Authors:
Author Name | Author Type | Creation Date |
TRC | Academic researcher, vector creator |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |