Vector: pWALIUM22

Number of plasmids with this backbone: 1
Name: pWALIUM22
Description: Drosophila expression vector. loxP, and GATEWAY recombination sites with MCS. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, p-transposase promoter with ftz intron and K10 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 11256
Properties: drosophila in vitro and in vivo expression
Vector Map: WALIUM22_map_seq.pdf
Vector Sequence: pWALIUM22.txt
Comments: Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacZ Alpha 463 531
primer site M13 forward 602 619
promoter T7 618 645
selectable marker white 682 4808
Cloning Site attB1 4851 5218
repressor binding site Gypsy Insulator 5230 5660
Cloning Site LoxP 5678 5711
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5718 5827
Cloning Site LoxP #2 5834 5867
Cloning Site 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5874 5983
promoter p-transposase 5990 6234
restriction site Nhe I 6286 6291
primer site M13 Forward #2 6317 6334
restriction site EcoRI 6903 6908
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 7009 7155
polyA site K10 7233 8609
repressor binding site Gypsy Insulator #2 8616 9046
primer site T3 9069 9088
primer site M13 reverse 9106 9126
bacterial operon LacO 9132 9154
bacterial origin ColE1 9494 10176
bacterial selection antibiotic AmpR 10274 10933

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments