Vector: pWALIUM10-roe

Number of plasmids with this backbone: 1
Name: pWALIUM10-roe
Description: Drosophila expression vector for Recombination cloning, Over-Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two Gypsy insulators, two 5xUAS, Hsp70 promoter, with ftz intron and SV40 poly A. White selection marker in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 11556
Properties: drosophila in vitro and in vivo expression
Vector Map: WALIUM10roe_map_seq.pdf
Vector Sequence: pWALIUM10-roe.txt
Comments: To amplify this vector you will need to use One Shot ccdB Survival 2 T1R Competent Cells(Invitrogen Cat. No. A10460), or other gateway competent cells. Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacZ Alpha 463 531
primer site M13 forward 602 619
promoter T7 618 645
selectable marker white 682 4808
Cloning Site attB1 4851 5218
ribosome binding site Gypsy Insulator 5230 5660
Cloning Site LoxP 5678 5711
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5718 5827
Cloning Site LoxP #2 5834 5867
enhancer 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 5874 5983
promoter Hsp70 70 kilodalton heat shock protein 5990 6249
primer site pUAST_Forward TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification 6177 6193
Cloning Marker attR1 A Gateway® cassette containing attR recombination sites flanking a ccdB gene and a chloramphenicol-resistance gene 6277 7988
bacterial selection antibiotic CmR 6514 7170
toxin ccdB 7515 7820
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 8046 8192
polyA site SV40 8216 8915
repressor binding site Gypsy Insulator #2 8922 9352
primer site T3 9369 9388
primer site M13 reverse 9406 9426
bacterial operon LacO 9432 9454
bacterial origin ColE1 9794 10476
bacterial selection antibiotic AmpR 10574 11233

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments