Vector: pVALIUM10-moe
Number of plasmids with this backbone: | 1 |
Name: | pVALIUM10-moe |
Description: | Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 7541 |
Properties: | drosophila in vitro and in vivo expression |
Vector Map: | VALIUM10moe_map_seq.pdf |
Vector Sequence: | pVALIUM10-moe.txt |
Comments: | Revised 4/25/2012 |
Type | Name | Description | Start Position | End Position |
bacterial origin | F1 origin | 138 | 444 | |
bacterial operon | LacZ | Alpha | 463 | 531 |
primer site | M13 forward | 602 | 619 | |
primer site | T7 | 618 | 645 | |
selectable marker | vermilion | 664 | 2543 | |
Cloning Site | attB1 | 2568 | 2935 | |
repressor binding site | Gypsy Insulator | 2947 | 3377 | |
Cloning Site | LoxP | 3395 | 3428 | |
enhancer | 5xUAS | UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription | 3435 | 3544 |
Cloning Site | LoxP #2 | 3551 | 3584 | |
enhancer | 5xUAS #2 | UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription | 3591 | 3700 |
promoter | Hsp70 | 70 kilodalton heat shock protein | 3707 | 3966 |
primer site | pUAST_Forward | TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification | 3894 | 3910 |
Cloning Site | MCS | 3967 | 3990 | |
intron | ftz | Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu | 4031 | 4177 |
polyA site | SV40 | 4201 | 4900 | |
repressor binding site | Gypsy Insulator #2 | 4907 | 5337 | |
primer site | T3 | 5354 | 5373 | |
primer site | M13 reverse | 5391 | 5411 | |
bacterial operon | LacO | 5417 | 5439 | |
bacterial origin | ColE1 | 5779 | 6461 | |
bacterial selection antibiotic | AmpR | 6559 | 7218 |
Authors:
Author Name | Author Type | Creation Date |
Norbert Perrimon | Academic researcher, vector PI | |
Jian-Quan Ni | Academic researcher, vector creator |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |