Vector Detail

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Vector: pVALIUM10-moe

Name:	pVALIUM10-moe
Description:	Drosophila expression vector for Multicloning site, Over-Expression (MOE). loxP sites and restriction sites for EcoRI, BgIII, and Xbal.Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, MCS, with ftz intron and SV40 polyA. Vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type:	bacterial plasmid
Form:	dsDNA
Size (bp)::	7541
Properties:	drosophila in vitro and in vivo expression
Vector Map:	VALIUM10moe_map_seq.pdf
Vector Sequence:	pVALIUM10-moe.txt
Comments:	Revised 4/25/2012

Type	Name	Description	Start Position	End Position
bacterial origin	F1 origin		138	444
bacterial operon	LacZ	Alpha	463	531
primer site	M13 forward		602	619
primer site	T7		618	645
selectable marker	vermilion		664	2543
Cloning Site	attB1		2568	2935
repressor binding site	Gypsy Insulator		2947	3377
Cloning Site	LoxP		3395	3428
enhancer	5xUAS	UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription	3435	3544
Cloning Site	LoxP #2		3551	3584
enhancer	5xUAS #2	UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription	3591	3700
promoter	Hsp70	70 kilodalton heat shock protein	3707	3966
primer site	pUAST_Forward	TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification	3894	3910
Cloning Site	MCS		3967	3990
intron	ftz	Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu	4031	4177
polyA site	SV40		4201	4900
repressor binding site	Gypsy Insulator #2		4907	5337
primer site	T3		5354	5373
primer site	M13 reverse		5391	5411
bacterial operon	LacO		5417	5439
bacterial origin	ColE1		5779	6461
bacterial selection antibiotic	AmpR		6559	7218

Authors:

Author Name	Author Type	Creation Date
Norbert Perrimon	Academic researcher, vector PI
Jian-Quan Ni	Academic researcher, vector creator

Publications:

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Comments