Vector: pVALIUM10-roe

Number of plasmids with this backbone: 1
Name: pVALIUM10-roe
Description: Drosophila expression vector for Recombination cloning, Over Expression (ROE). loxP, and GATEWAY recombination sites. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Two gypsy insulators, two 5xUAS, Hsp70 promoter, ftz intron, SV40 poly A, vermilion selection in drosophila, AmpR in bacteria. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 9240
Properties: drosophila in vitro and in vivo expression
Vector Map: VALIUM10roe_map_seq.pdf
Vector Sequence: pVALIUM10-roe.txt
Comments: To amplify this vector you will need to use One Shot ccdB Survival 2 T1R Competent Cells (Invitrogen Cat. No. A10460), or other GATEWAY competent cells. Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacZ Alpha 463 531
primer site M13 forward 602 619
promoter T7 618 645
selectable marker vermilion 664 2543
Cloning Site attB1 2568 2935
repressor binding site Gypsy Insulator 2947 3377
Cloning Site LoxP 3395 3428
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3435 3544
Cloning Site LoxP #2 3551 3584
enhancer 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3591 3700
promoter Hsp70 70 kilodalton heat shock protein 3707 3966
primer site pUAST_Forward TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification 3894 3910
Cloning Marker attR1 A Gateway® cassette containing attR recombination sites flanking a ccdB gene and a chloramphenicol-resistance gene 3994 5705
bacterial selection antibiotic CmR 4231 4887
toxin ccdB 5232 5537
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 5763 5909
polyA site SV40 5933 6632
repressor binding site Gypsy Insulator #2 6639 7069
primer site T3 7086 7105
primer site M13 reverse 7123 7143
bacterial operon LacO 7149 7171
bacterial origin ColE1 7510 8192
bacterial selection antibiotic AmpR 8291 8950

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments