Vector: pVALIUM22

Number of plasmids with this backbone: 1
Name: pVALIUM22
Description: Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI andEcRI method to clone shmiR. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html P-Trans promoter with ftz intron, two gypsy insulators,two 5xUAS, K10 localization element and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Specialty sequencing primer required to verify insert. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8973
Properties: drosophila in vitro and in vivo expression
Vector Map: VALIUM22_map_seq.pdf
Vector Sequence: pVALIUM22.txt
Comments: Revised 4/25/2012

Features:

Type Name Description Start Position End Position
bacterial origin F1 origin 138 444
bacterial operon LacZ Alpha 463 531
primer site M13 forward 602 619
promoter T7 618 645
selectable marker vermilion 664 2543
Cloning Site attB1 2568 2935
repressor binding site Gypsy Insulator 2947 3377
Cloning Site LoxP 3395 3428
enhancer 5xUAS UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3435 3544
Cloning Site LoxP #2 3551 3584
enhancer 5xUAS #2 UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription 3591 3700
promoter p-transposase 3707 3951
restriction site Nhe I 4003 4008
primer site M13 Forward #2 4034 4051
restriction site EcoRI 4620 4625
intron ftz Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu 4726 4872
localization element K10 transport/localization element (TLS) is responsible for the transport and anterior localization of K10 mRNA and acts to establish dorso-ventral polarity in the oocyte [1=PMID 8582290][2=PMID 11533666] 4950 6326
repressor binding site Gypsy Insulator #2 6333 6763
primer site T3 6786 6805
primer site M13 reverse 6823 6843
bacterial operon LacO 6849 6871
bacterial origin ColE1 7211 7893
bacterial selection antibiotic AmpR 7991 8650

Authors:

Author Name Author Type Creation Date
Norbert Perrimon Academic researcher, vector PI
Jian-Quan Ni Academic researcher, vector creator

Publications:

PMID Title
21460824 A genome-scale shRNA resource for transgenic RNAi in Drosophila.

Parent Vector:

Name Comments