Type |
Name |
Description |
Start Position |
End Position |
bacterial origin |
F1 origin |
|
138 |
444 |
bacterial operon |
LacZ |
alpha |
463 |
531 |
primer site |
M13 forward |
|
602 |
619 |
promoter |
T7 |
|
619 |
645 |
selectable marker |
vermilion |
|
664 |
2543 |
Cloning Site |
attB1 |
|
2568 |
2935 |
repressor binding site |
Gypsy Insulator |
|
2947 |
3377 |
Cloning Site |
LoxP |
|
3395 |
3428 |
enhancer |
5xUAS |
UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription |
3435 |
3544 |
Cloning Site |
LoxP #2 |
|
3551 |
3584 |
enhancer |
5xUAS #2 |
UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription |
3591 |
3700 |
promoter |
Hsp70 |
70 kilodalton heat shock protein |
3707 |
3966 |
primer site |
pUAST_Forward |
TCAACTGCAACTACTGA
hsp70(+60)f Recommended for insert verification |
3894 |
3910 |
restriction site |
Nhe I |
|
4018 |
4023 |
primer site |
M13 Forward #2 |
|
4049 |
4066 |
restriction site |
EcoRI |
|
4635 |
4640 |
intron |
ftz |
Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu |
4747 |
4893 |
viral gene |
SV40 |
poly A |
4917 |
5616 |
repressor binding site |
Gypsy Insulator #2 |
|
5623 |
6053 |
primer site |
T3 |
|
6069 |
6088 |
primer site |
M13 reverse |
|
6106 |
6126 |
bacterial operon |
LacO |
|
6132 |
6154 |
bacterial origin |
ColE1 |
|
6494 |
7176 |
bacterial selection antibiotic |
AmpR |
|
7275 |
7934 |