Vector Detail

After careful financial evaluations, the PlasmID repository will be increasing plasmid retrieval fees on October 1st, 2016. The new fee for the retrieval and validation of one clone will be $58 for Harvard and DF/HCC members, $75 for other academics and non-profits, and $90 for commercial users. Please be assured that we will always try to keep cost low while sustaining our operation at the same time.

Vector: pVALIUM20

Name:	pVALIUM20
Description:	Drosophila expression vector for high expression of hairpin insert. loxP, and GATEWAY recombination sites, NheI and EcoRI method to clone shmiR. Detailed procedure for small scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Hsp70 promoter with ftz intron, two gypsy insulators, 5xUAS and vermilion selection in drosophila. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA.
Synonyms:
Type:	bacterial plasmid
Form:	dsDNA
Size (bp)::	8190
Properties:	drosophila in vitro and in vivo expression
Vector Map:	VALIUM20_map_seq.pdf
Vector Sequence:	pVALIUM20.txt
Comments:	Revised 4/25/2012

Type	Name	Description	Start Position	End Position
bacterial origin	F1 origin		138	444
bacterial operon	LacZ	alpha	463	531
primer site	M13 forward		602	619
promoter	T7		619	645
selectable marker	vermilion		664	2543
Cloning Site	attB1		2568	2935
repressor binding site	Gypsy Insulator		2947	3377
Cloning Site	LoxP		3395	3428
enhancer	5xUAS	UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription	3435	3544
Cloning Site	LoxP #2		3551	3584
enhancer	5xUAS #2	UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription	3591	3700
promoter	Hsp70	70 kilodalton heat shock protein	3707	3966
primer site	pUAST_Forward	TCAACTGCAACTACTGA hsp70(+60)f Recommended for insert verification	3894	3910
restriction site	Nhe I		4018	4023
primer site	M13 Forward #2		4049	4066
restriction site	EcoRI		4635	4640
intron	ftz	Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu	4747	4893
viral gene	SV40	poly A	4917	5616
repressor binding site	Gypsy Insulator #2		5623	6053
primer site	T3		6069	6088
primer site	M13 reverse		6106	6126
bacterial operon	LacO		6132	6154
bacterial origin	ColE1		6494	7176
bacterial selection antibiotic	AmpR		7275	7934

Authors:

Author Name	Author Type	Creation Date
Norbert Perrimon	Academic researcher, vector PI
Jian-Quan Ni	Academic researcher, vector creator

Publications:

PMID	Title
21460824	A genome-scale shRNA resource for transgenic RNAi in Drosophila.

Parent Vector:

Name

Comments