Type |
Name |
Description |
Start Position |
End Position |
bacterial origin |
F1 origin |
|
138 |
444 |
bacterial operon |
LacZ |
alpha |
463 |
531 |
primer site |
M13 forward |
|
602 |
619 |
promoter |
T7 |
|
618 |
645 |
selectable marker |
vermilion |
|
664 |
2543 |
GATEWAY Cloning Site |
attB1 |
|
2568 |
2935 |
repressor binding site |
Gypsy Insulator |
|
2947 |
3377 |
Cloning Site |
LoxP |
|
3395 |
3428 |
enhancer |
5xUAS |
UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription |
3435 |
3544 |
Cloning Site |
LoxP #2 |
|
3551 |
3584 |
enhancer |
5xUAS #2 |
UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription |
3591 |
3700 |
promoter |
Hsp70 |
70 kilodalton heat shock protein |
3707 |
3966 |
primer site |
pUAST_Forward |
5' TCA ACT GCA ACT ACT GA 3'
hsp70(+60)f Recommended for insert verification |
3894 |
3910 |
Cloning Marker |
attR1 |
A Gateway® cassette containing attR recombination sites flanking a ccdB gene and a chloramphenicol-resistance gene |
3994 |
5705 |
bacterial selection antibiotic |
CmR |
|
4230 |
4887 |
Cloning Marker |
ccdB |
toxin |
5232 |
5537 |
intron |
ftz |
Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu |
5725 |
5871 |
Cloning Marker |
attR2 |
A Gateway® cassette containing attR recombination sites flanking a ccdB gene and a chloramphenicol-resistance gene |
5882 |
7586 |
Cloning Marker |
ccDB #2 |
|
6046 |
6352 |
bacterial selection antibiotic |
CmR #2 |
|
6696 |
7353 |
intron |
ftz #2 |
Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu |
7633 |
7779 |
polyA site |
SV40 |
|
7803 |
8502 |
repressor binding site |
Gypsy Insulator #2 |
|
8509 |
8939 |
primer site |
T3 |
|
8956 |
8975 |
primer site |
M13 reverse |
|
8993 |
9013 |
bacterial operon |
LacO |
|
9018 |
9040 |
bacterial origin |
ColE1 |
|
9381 |
10063 |
bacterial selection antibiotic |
AmpR |
|
10161 |
10820 |