Vector: pVALIUM1
Number of plasmids with this backbone: | 1 |
Name: | pVALIUM1 |
Description: | Drosophila expression vector for high expression of hairpin insert MCS, loxP, and attB recombination sites. Detailed procedure for smal scale cloning can be found on the TRiP website: http://flyrnai.org/TRiP-HOME.html Hsp70 promoter with white and ftz intron. AmpR in bacteria; vermilion marker in drosophila. Vector may recombine in bacteria and should be stored as DNA. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 6742 |
Properties: | drosophila in vitro and in vivo expression |
Vector Map: | VALIUM1_map_seq.pdf |
Vector Sequence: | pVALIUM1.txt |
Comments: | Revised 4/25/2012 |
Type | Name | Description | Start Position | End Position |
primer site | M13 forward | 602 | 619 | |
primer site | T7 | 618 | 645 | |
selectable marker | vermilion | 664 | 2543 | |
recombination site | attB1 | 2568 | 2935 | |
recombination site | LoxP | 2947 | 2980 | |
enhancer | 5xUAS | UAS (Upstream Activation Sequence), a short section of the promoter region, to which Gal4 specifically binds to activate gene transcription | 2987 | 3096 |
recombination site | LoxP #2 | 3103 | 3136 | |
enhancer | 5xUAS #2 | 3142 | 3252 | |
promoter | Hsp70 | 3259 | 3518 | |
primer site | pUAST_Forward | hsp70(+60)f Recommended for insert verification | 3366 | 3389 |
MCS | - | 3519 | 3625 | |
intron | white | 3547 | 3620 | |
intron | ftz | Involved in DNA binding. Also known as CG2047-PA,Fushi-Tarazu, Ultra-abdominal-like, ftz-PA, fushi tarazu | 3669 | 3815 |
polyA site | SV40 | 3839 | 4538 | |
primer site | T3 | 4555 | 4574 | |
primer site | M13 reverse | 4592 | 4612 | |
bacterial operon | LacO | 4618 | 4640 | |
bacterial origin | ColE1 | 4980 | 5662 | |
bacterial selection antibiotic | AmpR | 5760 | 6419 |
Authors:
Author Name | Author Type | Creation Date |
Norbert Perrimon | Academic researcher, vector PI | |
Jian-Quan Ni | Academic researcher, vector creator |
Publications:
PMID | Title |
18084299 | Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster |
Parent Vector:
Name | Comments |