Vector: pWE-p2A-mRFP

Number of plasmids with this backbone: 2
Name: pWE-p2A-mRFP
Description: Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Synonyms: pCW45-p2A-mRFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8144
Properties: mammalian expression
Vector Map: pCW45-2A-mRFP.pdf
Vector Sequence: pCW45-2A-mRFP.txt
Comments:

Features:

Type Name Description Start Position End Position
viral response element cppt 41 158
promoter CMV 207 987
Cloning Site LoxP 994 1027
splice site SA 1181 1232
Marker His 6x-His 1240 1257
IRES - 1282 1855
mammalian antibiotic BlastR 1869 2267
cleavage site 2a 2277 2336
Marker mRFP 2346 3020
viral response element WPRE 3030 3910
bacterial origin SV40 4143 4220
bacterial selection antibiotic AmpR 4280 5210
bacterial origin pUC ori 5365 5984
viral response element RRE 7510 7751

Authors:

Author Name Author Type Creation Date
Wilson Endege Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments