Vector: pWE-p2A-mRFP
Number of plasmids with this backbone: | 2 |
Name: | pWE-p2A-mRFP |
Description: | Mammalian expression vector for lentiviral work. Fusion format inserts are expressed with self excising fluorescent marker mRFP. LoxP cloning system. Viral components on backbone help expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
Synonyms: | pCW45-p2A-mRFP |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 8144 |
Properties: | mammalian expression |
Vector Map: | pCW45-2A-mRFP.pdf |
Vector Sequence: | pCW45-2A-mRFP.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
viral response element | cppt | 41 | 158 | |
promoter | CMV | 207 | 987 | |
Cloning Site | LoxP | 994 | 1027 | |
splice site | SA | 1181 | 1232 | |
Marker | His | 6x-His | 1240 | 1257 |
IRES | - | 1282 | 1855 | |
mammalian antibiotic | BlastR | 1869 | 2267 | |
cleavage site | 2a | 2277 | 2336 | |
Marker | mRFP | 2346 | 3020 | |
viral response element | WPRE | 3030 | 3910 | |
bacterial origin | SV40 | 4143 | 4220 | |
bacterial selection antibiotic | AmpR | 4280 | 5210 | |
bacterial origin | pUC ori | 5365 | 5984 | |
viral response element | RRE | 7510 | 7751 |
Authors:
Author Name | Author Type | Creation Date |
Wilson Endege | Academic researcher, vector creator |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |