Vector: pCW63
Number of plasmids with this backbone: | 1 |
Name: | pCW63 |
Description: | Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines. |
Synonyms: | pCW63-T7-Tag |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 7520 |
Properties: | mammalian expression |
Vector Map: | pCW63-T7-Tag.pdf |
Vector Sequence: | pCW63.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
viral response element | cppt | 41 | 158 | |
promoter | CMV | 207 | 987 | |
promoter | T7 | 996 | 1012 | |
Marker | HA | 1033 | 1062 | |
cleavage site | TEV | 1063 | 1083 | |
Marker | Flag | 1090 | 1113 | |
cleavage site | EK | 1099 | 1113 | |
Cloning Site | LoxP | 1120 | 1153 | |
splice site | SA | 1307 | 1358 | |
Marker | His | 1366 | 1383 | |
IRES | - | 1414 | 1987 | |
mammalian antibiotic | BlastR | 2001 | 2399 | |
viral response element | WPRE | 2406 | 2975 | |
bacterial origin | SV40 | 3519 | 3596 | |
bacterial selection antibiotic | AmpR | 3656 | 4586 | |
bacterial origin | pUC ori | 4741 | 5360 | |
viral response element | RRE | 6886 | 7127 |
Authors:
Author Name | Author Type | Creation Date |
Wilson Endege | Academic researcher, vector creator |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |