Vector: pCW63

Number of plasmids with this backbone: 1
Name: pCW63
Description: Mammalian expression vector for use in lentiviral work. LoxP site cloning. Fusion format inserts will be expressed with HA-Flag tag that can be systematically removed during protein purification with TEV and EK cleavage. Viral components on vector improve expression. Ampicillin resistance in bacterial cultures, blasticidin resistance in mammalian cell lines.
Synonyms: pCW63-T7-Tag
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7520
Properties: mammalian expression
Vector Map: pCW63-T7-Tag.pdf
Vector Sequence: pCW63.txt
Comments:

Features:

Type Name Description Start Position End Position
viral response element cppt 41 158
promoter CMV 207 987
promoter T7 996 1012
Marker HA 1033 1062
cleavage site TEV 1063 1083
Marker Flag 1090 1113
cleavage site EK 1099 1113
Cloning Site LoxP 1120 1153
splice site SA 1307 1358
Marker His 1366 1383
IRES - 1414 1987
mammalian antibiotic BlastR 2001 2399
viral response element WPRE 2406 2975
bacterial origin SV40 3519 3596
bacterial selection antibiotic AmpR 3656 4586
bacterial origin pUC ori 4741 5360
viral response element RRE 6886 7127

Authors:

Author Name Author Type Creation Date
Wilson Endege Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments