Vector: pWE3-F2

Number of plasmids with this backbone: 3
Name: pWE3-F2
Description: Mammalian expression vector for lentiviral work. Uses PHAGE recombinational cloning (compatible with GATEWAY). HA-FLAG tag added to frame 2 fusion format inserts during expression. Viral components on backbone improve expression in mammalian cell lines.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 9573
Properties: mammalian expression
Vector Map: pWE3-F2.pdf
Vector Sequence: pWE3-F2.txt
Comments: contains ccdB when no insert present must propagate in resistant E. coli strain such as DB3.1

Features:

Type Name Description Start Position End Position
viral response element cppt 41 158
promoter CMV 207 987
promoter T7 1015 1032
Cloning Site attR1 1063 1187
Cloning Marker ccdB 1616 1921
Cloning Marker CAT 2263 2922
Cloning Site attR2 3203 3303
Marker Flag 3359 3382
Marker HA 3395 3424
IRES - 3467 4040
mammalian antibiotic BlastR 4054 4452
viral response element WPRE 4459 5028
viral LTR 3' 5106 5339
bacterial origin SV40 5572 5649
promoter bla 5681 5778
bacterial selection antibiotic AmpR 5779 6639
bacterial origin pUC ori 6794 7413
promoter lac 7722 7748
viral response element RSV RSV-promoter 7865 8093
viral LTR 5' 8094 8429
viral response element RRE 8939 9180

Authors:

Author Name Author Type Creation Date
Wilson Endege Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments