Vector: pOTB7

Number of plasmids with this backbone: 7384
Name: pOTB7
Description: Gateway® or compatible clone with M13 forward and M13 reverse primers. Chloramphenicol resistance in bacteria. More information available at http://genecollections.nci.nih.gov/MGC/Vectors/prot_potb7
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 1815
Properties: Gateway
Vector Map: potb7.gif
Vector Sequence: pOTB7.txt
Comments: A set of thirty-six MGC libraries was made in this vector by Dr. Gerry Rubin, University of California, Berkeley CA. Briefly, the method was as follows: Poly A+RNA was reverse trancribed using an anchored oligo-dT primer with an Xho I site. Double stranded cDNA was prepared using a cDNA synthesis kit. The double stranded cDNA was ligated to Eco RI adaptors, digested with Xho I, size selected over a column, and ligated into the vector. The pOTB7 vector was constructed by Dr. Michael Brownstein, National Institute of Mental Health, NIH, Bethesda, MD.

Features:

Type Name Description Start Position End Position
primer site SP6 53 70
GATEWAY Cloning Site attB1 85 109
primer site M13 forward 111 127
primer site M13 reverse 250 232
GATEWAY Cloning Site attB2 253 277
promoter T7 311 293
bacterial origin pBR322 origin 411 1018
bacterial selection antibiotic chloramphenicol 1144 1803

Authors:

Author Name Author Type Creation Date
UCBerkeley/Rubin Academic researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments