Vector: pCR-XL-TOPO

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: pCR-XL-TOPO
Description: TOPO Cloning vector for bacterial expression; kanamycin resistance in bacteria
Synonyms: XL Topo
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3500
Properties: bacterial expression
Vector Map: xltopo.bmp
Vector Sequence: pCR_XL_TOPO.txt
Comments: This vector has both kanamycin and zeocin resistance

Features:

Type Name Description Start Position End Position
promoter lac 95 216
primer M13 reverse 205 221
promoter LacZ-alpha ORF 217 576
MCS MCS Some restriction sites may have been lost during cloning 248 399
TOPO recognition site TOPO Cloning Site 336 337
promoter T7 406 425
primer M13 forward 433 448
Fusion joint - 577 585
toxin ccdB Missing in clones with insert 586 888
bacterial selection antibiotic KanR 1237 2031
bacterial selection antibiotic zeocin 2238 2612
bacterial origin pUC ori 2680 3393

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial Vector

Publications:

PMID Title

Parent Vector:

Name Comments