Vector: pCR-XL-TOPO
This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions. |
Name: | pCR-XL-TOPO |
Description: | TOPO Cloning vector for bacterial expression; kanamycin resistance in bacteria |
Synonyms: | XL Topo |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 3500 |
Properties: | bacterial expression |
Vector Map: | xltopo.bmp |
Vector Sequence: | pCR_XL_TOPO.txt |
Comments: | This vector has both kanamycin and zeocin resistance |
Type | Name | Description | Start Position | End Position |
promoter | lac | 95 | 216 | |
primer | M13 reverse | 205 | 221 | |
promoter | LacZ-alpha ORF | 217 | 576 | |
MCS | MCS | Some restriction sites may have been lost during cloning | 248 | 399 |
TOPO recognition site | TOPO Cloning Site | 336 | 337 | |
promoter | T7 | 406 | 425 | |
primer | M13 forward | 433 | 448 | |
Fusion joint | - | 577 | 585 | |
toxin | ccdB | Missing in clones with insert | 586 | 888 |
bacterial selection antibiotic | KanR | 1237 | 2031 | |
bacterial selection antibiotic | zeocin | 2238 | 2612 | |
bacterial origin | pUC ori | 2680 | 3393 |
Authors:
Author Name | Author Type | Creation Date |
Invitrogen | Commercial Vector |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |