Vector: pCR4-TOPO

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: pCR4-TOPO
Description: TOPO Cloning vector with ampicillin resistance in bacteria.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3956
Properties: TOPO Cloning
Vector Map: pCR4TopoTA.bmp
Vector Sequence: CR4Topo.txt
Comments:

Features:

Type Name Description Start Position End Position
promoter lac 2 216
primer M13 reverse 205 221
toxin ccdB 217 810
primer T3 243 262
TOPO recognition site TOPO Cloning Site 294 295
promoter T7 328 347
primer M13 forward 355 370
bacterial selection antibiotic KanR 1159 1953
bacterial selection antibiotic AmpR 2203 3063
bacterial origin pUC ori 3161 3834

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial Vector

Publications:

PMID Title

Parent Vector:

Name Comments