Vector: pCMV-SPORT6.ccdb
Number of plasmids with this backbone: | 174 |
Name: | pCMV-SPORT6.ccdb |
Description: | Gateway vector with CMV promoter and ampicillin resistance in bacteria |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 4807 |
Properties: | mammalian expression |
Vector Map: | pCMV-Sport6ccdB.gif |
Vector Sequence: | pCMV_Sport_6_ccdb.txt |
Comments: | also known as pCMV-SPORT6.1.ccdb |
Type | Name | Description | Start Position | End Position |
restriction site | Cla I | 124 | 0 | |
restriction site | Hpa I | 260 | 0 | |
promoter | CMV | Reverse primer site | 334 | 355 |
restriction site | Nhe I | 638 | 0 | |
primer site | M13 forward | 664 | 680 | |
promoter | T7 | 692 | 708 | |
recombination site | attB2 | 708 | 735 | |
restriction site | Mlu I | 737 | 0 | |
restriction site | HindIII | 743 | 0 | |
restriction site | ApaI | 753 | 0 | |
restriction site | Xhol | 755 | 0 | |
restriction site | BamHI | 761 | 0 | |
restriction site | EagI | 774 | 0 | |
restriction site | Not I | 774 | 0 | |
restriction site | BssHII | 822 | 0 | |
toxin | ccdB | 822 | 1206 | |
restriction site | SrfI | 983 | 0 | |
restriction site | Sal I | 1206 | 0 | |
restriction site | EcoRV | 1214 | 0 | |
restriction site | EcoRI | 1222 | 0 | |
restriction site | Rsr II | 1229 | 0 | |
restriction site | Age I | 1231 | 0 | |
restriction site | Acc65I | 1234 | 0 | |
restriction site | Kpn I | 1238 | 0 | |
recombination site | attB1 | 1239 | 1262 | |
primer | SP6 | Short version | 1255 | 1281 |
restriction site | Stu I | 1286 | 0 | |
primer | M13 reverse | 1291 | 1308 | |
restriction site | SAC II | 1333 | 0 | |
restriction site | SAC I | 1439 | 0 | |
restriction site | Nco I | 1639 | 0 | |
restriction site | SnaB I | 1663 | 0 | |
restriction site | Nsi I | 1950 | 0 | |
bacterial selection antibiotic | AmpR | 3271 | 3956 | |
restriction site | AspEI | 3277 | 0 | |
restriction site | Sca I | 3757 | 0 | |
restriction site | NaeI | 4679 | 0 |
Authors:
Author Name | Author Type | Creation Date |
Invitrogen | Commercial Vector |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |