Vector: pCMV-SPORT6

Number of plasmids with this backbone: 15745
Name: pCMV-SPORT6
Description: Clone with T7, SP6, and CMV promoters and LoxP site. ampicillin resistance in bacteria
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4396
Properties: loxP
Vector Map: pcmv-sport6.gif
Vector Sequence: pCMV_Sport_6.txt
Comments: An MluI site is introduced upon ligation of a cDNA insert. "pCMV-SPORT6 Library Method Overview: Prior to January 2001, Life Technologies, Invitrogen Corp., Carlsbad, CA, constructed non-normalized (standard) libraries in the pCMV-SPORT6 vector. Briefly, the library method was as follows: First strand cDNA was synthesized from polyA+mRNA using Invitrogen Superscript II RT and an oligo-dT primer containing a Not I site. Second strand cDNA was synthesized. Sal I adapters were ligated onto the double stranded cDNA which was then digested with Not I creating 5'-Sal I/3'- Not I inserts. This cDNA was size-fractionated on a column and ligated into the pCMV-Sport6 vector." http://mgc.nci.nih.gov/Vectors/prot_pcmv-sport6

Features:

Type Name Description Start Position End Position
restriction site Cla I 122 0
restriction site Hpa I 257 0
restriction site Mun I 268 0
restriction site Mam I 356 0
restriction site Bcl I 361 0
restriction site Nhe I 637 0
primer site M13 forward 664 680
promoter T7 691 709
GATEWAY Cloning Site attB2 735 711
restriction site Mlu I Multiple Cloning Site with Kpn 736 0
restriction site Kpn I Multiple Cloning Site with Mlu I 822 0
GATEWAY Cloning Site attB1 852 828
promoter SP6 871 854
restriction site Stu I 872 0
primer site M13 reverse 917 895
restriction site Sst II 918 0
restriction site Avr II 924 0
restriction site Bbs I 960 0
restriction site Esp3 I 992 0
promoter CMV 1074 1003
restriction site Nco I 1227 0
restriction site SnaB I 1249 0
restriction site Nsi I 1534 0
restriction site Sap I 1807 0
restriction site BspLU 11 I 1930 0
restriction site AlwN I 2341 0
restriction site Eam1105 I 2860 0
restriction site Bsa I 2932 0
restriction site Sca I 3343 0
restriction site Bcg I 3381 0
restriction site Xmn I 3460 0
bacterial selection antibiotic AmpR 3652 2792
restriction site PflM I 3846 0
restriction site Dra III 4159 0
restriction site NgoA IV 4265 0

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial Vector

Publications:

PMID Title

Parent Vector:

Name Comments