Vector: pCMV-SPORT6
Number of plasmids with this backbone: | 15745 |
Name: | pCMV-SPORT6 |
Description: | Clone with T7, SP6, and CMV promoters and LoxP site. ampicillin resistance in bacteria |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 4396 |
Properties: | loxP |
Vector Map: | pcmv-sport6.gif |
Vector Sequence: | pCMV_Sport_6.txt |
Comments: | An MluI site is introduced upon ligation of a cDNA insert. "pCMV-SPORT6 Library Method Overview: Prior to January 2001, Life Technologies, Invitrogen Corp., Carlsbad, CA, constructed non-normalized (standard) libraries in the pCMV-SPORT6 vector. Briefly, the library method was as follows: First strand cDNA was synthesized from polyA+mRNA using Invitrogen Superscript II RT and an oligo-dT primer containing a Not I site. Second strand cDNA was synthesized. Sal I adapters were ligated onto the double stranded cDNA which was then digested with Not I creating 5'-Sal I/3'- Not I inserts. This cDNA was size-fractionated on a column and ligated into the pCMV-Sport6 vector." http://mgc.nci.nih.gov/Vectors/prot_pcmv-sport6 |
Type | Name | Description | Start Position | End Position |
restriction site | Cla I | 122 | 0 | |
restriction site | Hpa I | 257 | 0 | |
restriction site | Mun I | 268 | 0 | |
restriction site | Mam I | 356 | 0 | |
restriction site | Bcl I | 361 | 0 | |
restriction site | Nhe I | 637 | 0 | |
primer site | M13 forward | 664 | 680 | |
promoter | T7 | 691 | 709 | |
GATEWAY Cloning Site | attB2 | 735 | 711 | |
restriction site | Mlu I | Multiple Cloning Site with Kpn | 736 | 0 |
restriction site | Kpn I | Multiple Cloning Site with Mlu I | 822 | 0 |
GATEWAY Cloning Site | attB1 | 852 | 828 | |
promoter | SP6 | 871 | 854 | |
restriction site | Stu I | 872 | 0 | |
primer site | M13 reverse | 917 | 895 | |
restriction site | Sst II | 918 | 0 | |
restriction site | Avr II | 924 | 0 | |
restriction site | Bbs I | 960 | 0 | |
restriction site | Esp3 I | 992 | 0 | |
promoter | CMV | 1074 | 1003 | |
restriction site | Nco I | 1227 | 0 | |
restriction site | SnaB I | 1249 | 0 | |
restriction site | Nsi I | 1534 | 0 | |
restriction site | Sap I | 1807 | 0 | |
restriction site | BspLU 11 I | 1930 | 0 | |
restriction site | AlwN I | 2341 | 0 | |
restriction site | Eam1105 I | 2860 | 0 | |
restriction site | Bsa I | 2932 | 0 | |
restriction site | Sca I | 3343 | 0 | |
restriction site | Bcg I | 3381 | 0 | |
restriction site | Xmn I | 3460 | 0 | |
bacterial selection antibiotic | AmpR | 3652 | 2792 | |
restriction site | PflM I | 3846 | 0 | |
restriction site | Dra III | 4159 | 0 | |
restriction site | NgoA IV | 4265 | 0 |
Authors:
Author Name | Author Type | Creation Date |
Invitrogen | Commercial Vector |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |