Vector: pACT2

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: pACT2
Description: Yeast two-hybrid expression vector with ADH1 promoter, makes Gal4 Act fusion, LoxP sites; amp resistance (LEU2 for yeast); restriction enzyme and/or recombinational cloning.
Synonyms: pACT-2
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8117
Properties: loxP, recombinational cloning, two-hybrid, yeast expression, yeast shuttle
Vector Map: pACT2.pdf
Vector Sequence: pACT2.txt
Comments:

Features:

Type Name Description Start Position End Position
yeast origin 2 micron Yeast two micron origin 1 2057
selectable marker LEU2 LEU2 yeast nutritional marker 2474 3568
recombination site LoxP site 1 loxP site 4268 4327
recombination site loxP site 2 loxP site 4367 4412
trxn termination sequence ADH1 term ADH1 terminator 4415 4742
tag HA HA epitope tag (fused with Gal4 domain) 5042 5068
tag Gal4 Act Gal4 activation domain polypeptide CDS 5081 5488
promoter ADH1 pr ADH1 promoter 5504 5901
bacterial origin pBR322 ori pBR322-type ColE1 origin of replication 6336 6979
selectable marker ampR ampicillin resistance gene 7127 7987

Authors:

Author Name Author Type Creation Date
BD Biosciences Clontech Vector supplier

Publications:

PMID Title
8384581 The retinoblastoma protein associates with the protein phosphatase type I catalytic subunit

Parent Vector:

Name Comments