Vector: pACT2
This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions. |
Name: | pACT2 |
Description: | Yeast two-hybrid expression vector with ADH1 promoter, makes Gal4 Act fusion, LoxP sites; amp resistance (LEU2 for yeast); restriction enzyme and/or recombinational cloning. |
Synonyms: | pACT-2 |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 8117 |
Properties: | loxP, recombinational cloning, two-hybrid, yeast expression, yeast shuttle |
Vector Map: | pACT2.pdf |
Vector Sequence: | pACT2.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
yeast origin | 2 micron | Yeast two micron origin | 1 | 2057 |
selectable marker | LEU2 | LEU2 yeast nutritional marker | 2474 | 3568 |
recombination site | LoxP site 1 | loxP site | 4268 | 4327 |
recombination site | loxP site 2 | loxP site | 4367 | 4412 |
trxn termination sequence | ADH1 term | ADH1 terminator | 4415 | 4742 |
tag | HA | HA epitope tag (fused with Gal4 domain) | 5042 | 5068 |
tag | Gal4 Act | Gal4 activation domain polypeptide CDS | 5081 | 5488 |
promoter | ADH1 pr | ADH1 promoter | 5504 | 5901 |
bacterial origin | pBR322 ori | pBR322-type ColE1 origin of replication | 6336 | 6979 |
selectable marker | ampR | ampicillin resistance gene | 7127 | 7987 |
Authors:
Author Name | Author Type | Creation Date |
BD Biosciences Clontech | Vector supplier |
Publications:
PMID | Title |
8384581 | The retinoblastoma protein associates with the protein phosphatase type I catalytic subunit |
Parent Vector:
Name | Comments |