Vector: pCW62

Number of plasmids with this backbone: 15
Name: pCW62
Description: Lentiviral cDNA expression vector, with C-terminal 6xHis tag and N-terminal HA-Flag tag separated by a TEV protease cleavage site; Ampicillin and chloromphenical resistance in bacteria; blasticidin resistance in mammalian cells; CRE-Lox recombinational or restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7520
Properties: mammalian expression
Vector Map: pCW62-Map.pdf
Vector Sequence: pCW62-DNA-Sequence.dat
Comments:

Features:

Type Name Description Start Position End Position
selectable marker AmpR Ampicillin resistance gene 0 0
selectable marker CmR Chloromphenical resistance gene 0 0
bacterial origin pUC ori pUC bacterial origin of replication 0 0
recombination site LoxP LoxP recombination site 0 0
promoter T7 T7 promoter 0 0
selectable marker BlastR Blastocydin resistance gene for mammalian selection 0 0
tag His C terminal 6xHis tag 0 0
tag Flag N-terminal HA-Flag tandem tag (separated by TEV protease site) 0 0
protease cleavage site TEV TEV protease cleavage site (separates the HA-Flag tandem tag) 0 0
promoter CMV CMV promoter 0 0

Authors:

Author Name Author Type Creation Date
John Doench Academic researcher, vector creator 2009-05-21 10:54:44.0
Ed Harlow Academic researcher, vector PI 2009-05-21 10:54:44.0
Wilson Endege Academic researcher, vector creator 2009-05-21 10:54:44.0

Publications:

PMID Title

Parent Vector:

Name Comments