Vector: ZM552

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: ZM552
Description: Yeast expression vectorn with N-terminal 3X V5 epitope tag; ampicillin and chloramphenicol resistance in bacteria; LEU2 resistance in yeast; Gateway cloning.
Synonyms: ZM552
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8302
Properties: Gateway, with tag/fusion/marker, yeast expression
Vector Map:
Vector Sequence: ZM552.txt
Comments: ZM511 Stu1 vector plus Gateway attR frame B cassette EcoRV fragment; same as ZM515

Features:

Type Name Description Start Position End Position
selectable marker AmpR ampcillin resistance 0 0
selectable marker ChlR chloramphenicol resistance 0 0
selectable marker LEU2 LEU2 selectable marker in yeast 0 0
tag V5 N-terminal triple V5 tag 0 0
yeast centromere CEN yeast centromere 0 0
primer site M13R M13 reverse sequencing primer 0 0
promoter CMD1 CMD1 promoter 0 0
trxn termination sequence CYC1 CYC1 terminator 0 0
yeast ARS ARS ARS yeast origin of replication 0 0
primer site M13F M13 forward sequencing primer 0 0

Authors:

Author Name Author Type Creation Date
Zarmik Moqtaderi Academic Researcher, vector author

Publications:

PMID Title

Parent Vector:

Name Comments