Vector: pMCSG7

Number of plasmids with this backbone: 51
Name: pMCSG7
Description: Bacterial expresson vector with T7 promoter, adds N-terminal His tag and TEV protease site; amp resistance; ligation independent cloning (LIC).
Synonyms: pMCSG-7
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 0
Properties: bacterial expression, in vitro transcription, ligation independent cloning (LIC), with tag/fusion/marker
Vector Map: pMCSG7circ.jpg
Vector Sequence: pMCSG7.gb
Comments: pET21 derivative; for routine HTP purification

Features:

Type Name Description Start Position End Position
MCS LIC ligation-independent cloning (LIC) region 0 0
tag N-His N-terminal His tag cds 0 0
bacterial origin ori ColE1 pBR322-type bacterial origin of replication 0 0
selectable marker ampR ampicillin resistance gene 0 0
protease cleavage site TEV TEV cleavage site cds 0 0
ribosome binding site RBS ribosome binding site 0 0
promoter T7 T7 promoter 0 0
trxn termination sequence T7 term T7 terminator sequence 0 0
tag C-His C-terminal his tag cds 0 0

Authors:

Author Name Author Type Creation Date
MCSG NIGMS Protein Structure Initiative Site

Publications:

PMID Title
12071693 A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site

Parent Vector:

Name Comments