Vector: pPRIME-TREX-GFP-recipient
Number of plasmids with this backbone: | 1 |
Name: | pPRIME-TREX-GFP-recipient |
Description: | Lentiviral expression vector for shRNA expression with inducible (TREX) promoter, fluorescent (GFP marker); amp resistance; MAGIC bacterial mating cloning. |
Synonyms: | pPRIME-TREX-GFP-PheS-recipient, p192 |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 8724 |
Properties: | MAGIC mating system, RNA interference, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker |
Vector Map: | p192.JPG |
Vector Sequence: | p192.txt |
Comments: | MAGIC mating recipient for PRIME approach (Elledge Lab) |
Type | Name | Description | Start Position | End Position |
selectable marker | ampR | ampicillin resistance gene | 0 | 0 |
bacterial origin | ori | bacterial origin of replication (inferred) | 0 | 0 |
viral LTR | LTR | viral LTR | 835 | 1509 |
viral response element | HIV RRE | HIV Rev Response Element | 1539 | 2396 |
promoter | TREX | TET eukaryotic promoter (2X Tet operator sequences) | 2581 | 3288 |
fluorescent marker | GFP | GFP | 3302 | 4022 |
negative selection marker | PheS cassette | Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 | 4069 | 5490 |
RNA regulatory element | WRE | woodchuck hepatitis B virus RNA regulatory element | 5653 | 6242 |
viral LTR | LTR | viral LTR | 6262 | 6951 |
Authors:
Author Name | Author Type | Creation Date |
Steve Elledge | Academic researcher, vector PI | |
Frank Stegmeier | Academic researcher, vector donor and author |
Publications:
PMID | Title |
16141338 | A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells |
15731760 | MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules |
Parent Vector:
Name | Comments |