Vector: pPRIME-TREX-GFP-recipient

Number of plasmids with this backbone: 1
Name: pPRIME-TREX-GFP-recipient
Description: Lentiviral expression vector for shRNA expression with inducible (TREX) promoter, fluorescent (GFP marker); amp resistance; MAGIC bacterial mating cloning.
Synonyms: pPRIME-TREX-GFP-PheS-recipient, p192
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8724
Properties: MAGIC mating system, RNA interference, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Vector Map: p192.JPG
Vector Sequence: p192.txt
Comments: MAGIC mating recipient for PRIME approach (Elledge Lab)

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
viral LTR LTR viral LTR 835 1509
viral response element HIV RRE HIV Rev Response Element 1539 2396
promoter TREX TET eukaryotic promoter (2X Tet operator sequences) 2581 3288
fluorescent marker GFP GFP 3302 4022
negative selection marker PheS cassette Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 4069 5490
RNA regulatory element WRE woodchuck hepatitis B virus RNA regulatory element 5653 6242
viral LTR LTR viral LTR 6262 6951

Authors:

Author Name Author Type Creation Date
Steve Elledge Academic researcher, vector PI
Frank Stegmeier Academic researcher, vector donor and author

Publications:

PMID Title
16141338 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Parent Vector:

Name Comments