Vector: pPRIME-CMV-LNGFR-recipient

Number of plasmids with this backbone: 1
Name: pPRIME-CMV-LNGFR-recipient
Description: Lentiviral expression vector for shRNA expression with cell-surface marker; amp resistance; MAGIC bacterial mating cloning.
Synonyms: pPRIME-CMV-LNGFR-PheS-recipient, pPRIME-CMV-NGFR-PheS-recipient, pPRIME-CMV-NGFR-recipient, p211
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8744
Properties: MAGIC mating system, RNA interference, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector
Vector Map: p211.JPG
Vector Sequence: p211.txt
Comments: MAGIC mating recipient for PRIME approach (Elledge Lab)

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
viral LTR LTR viral LTR 835 1509
viral response element HIV RRE HIV Rev Response Element 1539 2396
promoter CMV pr CMV promoter 2589 3197
cell surface marker NGFR NGFR cell surface marker ORF 3216 4040
negative selection marker PheS cassette Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 4089 5510
RNA regulatory element WRE woodchuck hepatitis B virus RNA regulatory element 5673 6262
viral LTR LTR viral LTR 6282 6971

Authors:

Author Name Author Type Creation Date
Steve Elledge Academic researcher, vector PI
Frank Stegmeier Academic researcher, vector donor and author

Publications:

PMID Title
16141338 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Parent Vector:

Name Comments