Vector: pPRIME-CMV-dsRed-recipient

Number of plasmids with this backbone: 1
Name: pPRIME-CMV-dsRed-recipient
Description: Lentiviral expression vector for shRNA expression with fluorescent (DsRed) marker; amp resistance; MAGIC bacterial mating cloning.
Synonyms: pPRIME-CMV-dsRed-PheS-recipient, p209
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8593
Properties: MAGIC mating system, RNA interference, fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Vector Map: p209.JPG
Vector Sequence: p209.txt
Comments: MAGIC mating recipient for PRIME approach (Elledge Lab)

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
viral LTR LTR viral LTR 835 1509
viral response element HIV RRE HIV Rev Response Element 1539 2396
promoter CMV pr CMV promoter 2589 3197
fluorescent marker dsRed dsRed fluorescent protein coding region 3212 3889
negative selection marker PheS cassette Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 3938 5359
RNA regulatory element WRE woodchuck hepatitis B virus RNA regulatory element 5522 6111
viral LTR LTR viral LTR 6131 6820

Authors:

Author Name Author Type Creation Date
Steve Elledge Academic researcher, vector PI
Frank Stegmeier Academic researcher, vector donor and author

Publications:

PMID Title
16141338 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Parent Vector:

Name Comments