Vector: pET28a

Number of plasmids with this backbone: 4
Name: pET28a
Description: Bacterial expression vector with T7lac promoter, adds N-terminal His tag, thrombin cleavage site, internal T7 epitope tag, C-terminal His tag; kanamycin resistance; restriction enzyme cloning.
Synonyms: pET28, pET-28a
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 0
Properties: bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Vector Map: pET28.pdf
Vector Sequence:


Type Name Description Start Position End Position
trxn termination sequence T7 term T7 termination sequence 26 72
MCS MCS MCS (XhoI, NotI, EagI, HindIII, SalI, SacI, EcoRI, BamHI, NheI, NdeI, NcoI) 158 203
tag T7 tag T7 tag cds 207 239
tag His tag His tag cds (6xHis) 270 287
promoter T7 T7 promoter 370 386
repressor protein gene lacI cds lacI coding sequence (lacI cds) 773 1852
bacterial origin ori bacterial origin of replication 3286 0
selectable marker kanR kanamycin resistance gene 3995 4807
ssDNA origin f1 ori f1 origin 4903 5358


Author Name Author Type Creation Date
MCSG NIGMS Protein Structure Initiative Site


PMID Title

Parent Vector:

Name Comments