Vector: pPRIME-TET-GFP-recipient

Number of plasmids with this backbone: 1
Name: pPRIME-TET-GFP-recipient
Description: Lentiviral expression vector for shRNA expression with inducible (Tet) promoter, fluorescent (GFP) marker; amp resistance; MAGIC bacterial mating cloning.
Synonyms: pPRIME-TET-GFP-PheS-recipient, p126
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8350
Properties: MAGIC mating system, RNA interference, fluorescent marker, inducible expression, lentiviral infection, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Vector Map: p126.JPG
Vector Sequence: p126.txt
Comments: MAGIC mating recipient for PRIME approach (Elledge Lab)

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
viral LTR LTR viral LTR 835 1509
viral response element HIV RRE HIV Rev Response Element 1539 2396
promoter TET pr TET eukaryotic promoter 2581 2914
fluorescent marker GFP GFP 2928 3648
negative selection marker PheS cassette Includes conditional dominant Cl-Phe sensitive marker pheS Gly294 3695 5116
RNA regulatory element WRE woodchuck hepatitis B virus RNA regulatory element 5279 5868
viral LTR LTR viral LTR 5888 6577

Authors:

Author Name Author Type Creation Date
Steve Elledge Academic researcher, vector PI
Frank Stegmeier Academic researcher, vector donor and author

Publications:

PMID Title
16141338 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells
15731760 MAGIC, an in vivo genetic method for the rapid construction of recombinant DNA molecules

Parent Vector:

Name Comments