Vector: pBY011

Number of plasmids with this backbone: 5529
Name: pBY011
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7920
Properties: Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Vector Map: pBY011_map.pdf
Vector Sequence: pBY011.txt
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
yeast origin ARS1 ARS1 yeast origin of replication 0 0
recombination site attR1 "attR recombination site 1, lost or altered in recombined (with insert) form" 0 0
recombination site attR2 "attR recombination site 2, lost or altered in recombined (with insert) form" 0 0
selectable marker URA3 URA3 auxotrophy selectable marker (yeast) 0 0
yeast centromere CEN4 yeast centromeric sequence 0 0
selectable marker chlR chloramphenicol resistance gene LOST in recombined (with insert) form 0 0
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 0 0
promoter Gal1-10 promoter Gal1-10 yeast promoter 0 0
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 0 0

Authors:

Author Name Author Type Creation Date
Bhullar Academic researcher, vector creator

Publications:

PMID Title

Parent Vector:

Name Comments
YCplac33 Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.