Vector: pBY011

Number of plasmids with this backbone: 5529
Name: pBY011
Description: Yeast expression vector with ARS1, CEN4, Gal1-10 promoter; amp resistance (URA3 for yeast); recombinational cloning.
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7920
Properties: Gateway, acceptor (destination), inducible expression, yeast expression, yeast shuttle
Vector Map: pBY011_map.pdf
Vector Sequence: pBY011.txt
Comments: This is a bacteria-yeast shuttle vector useful for expressing cDNAs in yeast that includes an auxotrophic marker for selection in yeast.


Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
yeast origin ARS1 ARS1 yeast origin of replication 0 0
recombination site attR1 "attR recombination site 1, lost or altered in recombined (with insert) form" 0 0
recombination site attR2 "attR recombination site 2, lost or altered in recombined (with insert) form" 0 0
selectable marker URA3 URA3 auxotrophy selectable marker (yeast) 0 0
yeast centromere CEN4 yeast centromeric sequence 0 0
selectable marker chlR chloramphenicol resistance gene LOST in recombined (with insert) form 0 0
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 0 0
promoter Gal1-10 promoter Gal1-10 yeast promoter 0 0
negative selection marker ccdB ccdB negative selection marker gene LOST in recombined (with insert) form 0 0


Author Name Author Type Creation Date
Bhullar Academic researcher, vector creator


PMID Title

Parent Vector:

Name Comments
YCplac33 Parent vector is a CEN4, URA3, ampR, ColE1 vector from R.D. Geitz described in Gene 74(2) 5270534 (1988). The Gal1-10 promoter and the Gateway-type attR recombination cassette (reference frame A, see information at the Invitrogen website) were inserted into the parent to create pBY011.