Vector: pENTR223.1

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: pENTR223.1
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Synonyms: pENTR223-star-Sfi, pDONR-223.1
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 4311
Properties: Gateway, donor (entry), recombinational cloning
Vector Map: pENTR223-1-map.doc
Vector Sequence: pENTR223_1.txt
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.

Features:

Type Name Description Start Position End Position
recombination site attL1 attL1 recombination site 0 0
recombination site attL2 attL2 recombination site 0 0
negative selection marker ccdB ccdB (no insert form only; lost in with insert form) 0 0
selectable marker chlR chlR (no insert form only; lost in with insert form) 0 0
primer site T7 Rev T7 Reverse primer for insert sequencing 0 0
MCS MCS MCS (Sfi sites flank the cassette present only in no insert form) 0 0
bacterial origin ori bacterial origin of replication 0 0
selectable marker SpnR spectinomycin resistance gene 0 0
primer site M13 F M13 forward primer for insert sequencing 0 0

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial Vendor

Publications:

PMID Title

Parent Vector:

Name Comments