Vector: pENTR223

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact plasmidhelp@hms.harvard.edu if you have any questions.
Name: pENTR223
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; spectinomycin resistance; recombinational cloning.
Synonyms: pDONR223, pENTR223-XnCDSYn
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 2815
Properties: recombinational cloning
Vector Map: pDONR223.1.gif
Vector Sequence: pENTR223-seq.doc
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml; also see supplemental figure 1 of the paper PubMed ID 15489335.

Features:

Type Name Description Start Position End Position
gene fragment pas 5p stem of pBR primose assembly site 63 73
gene fragment pas prime 3p stem of pBR pas(L) 105 116
trxn termination sequence T1-T2 rrnBT1 and T2 terminators 510 224
recombination site attL1 attL1 recombination site 570 665
recombination site attL2 attL2 recombination site 793 696
selectable marker SpnR spectinomycin resistance gene 1062 2072
bacterial origin ori bacterial origin of replication 2220 2753

Authors:

Author Name Author Type Creation Date
Mark Vidal Academic researcher, vector PI

Publications:

PMID Title
15489335 Human ORFeome Version 1.1: A Platform for Reverse Proteomics

Parent Vector:

Name Comments