Vector: pHR-MMPCreGFP

Number of plasmids with this backbone: 1
Name: pHR-MMPCreGFP
Description: Self-excising mammalian retroviral vector with Cre-GFP enzyme-marker and LoxP sites; ampicillin resistance
Synonyms: pHR-MMP-Cre-GFP, pHR-MMP-CreGFP, pHit-and-Run, pMMP-Hit-and-run-CreGFP, pMMPHit and Run CreGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8142
Properties: fluorescent marker, loxP, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Vector Map: pHR-MMPCreGFP.pdf
Vector Sequence: pHR-MMPCreGFP.txt
Comments: Vector donor notes fussy to grow; try RecA+ strain and/or growth for 1 hour without selection to give it a boost.

Features:

Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication (inferred) 0 0
recombination site loxP loxP recombination site (LOX 511) 0 0
viral LTR U3-R-U5 U3-R-U5 LTR for retroviral integration 0 0
Cre coding region Cre-GFP Cre-GFP fusion protein coding region 2428 4209
fluorescent marker Cre-GFP Cre-GFP fusion protein coding region 2428 4209
selectable marker ampR ampicillin resistance gene 6687 7544

Authors:

Author Name Author Type Creation Date
Daniel Silver Academic researcher, vector donor

Publications:

PMID Title
11511376 Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity

Parent Vector:

Name Comments