Vector: pHR-MMPCreGFP

This vector is NOT available from the PlasmID Repository and is provided here only as a reference. Please contact if you have any questions.
Description: Self-excising mammalian retroviral vector with Cre-GFP enzyme-marker and LoxP sites; ampicillin resistance
Synonyms: pHR-MMP-Cre-GFP, pHR-MMP-CreGFP, pHit-and-Run, pMMP-Hit-and-run-CreGFP, pMMPHit and Run CreGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 8142
Properties: fluorescent marker, loxP, mammalian expression, mammalian transduction, retroviral vector, with tag/fusion/marker
Vector Map: pHR-MMPCreGFP.pdf
Vector Sequence: pHR-MMPCreGFP.txt
Comments: Vector donor notes fussy to grow; try RecA+ strain and/or growth for 1 hour without selection to give it a boost.


Type Name Description Start Position End Position
bacterial origin ori bacterial origin of replication (inferred) 0 0
recombination site loxP loxP recombination site (LOX 511) 0 0
viral LTR U3-R-U5 U3-R-U5 LTR for retroviral integration 0 0
Cre coding region Cre-GFP Cre-GFP fusion protein coding region 2428 4209
fluorescent marker Cre-GFP Cre-GFP fusion protein coding region 2428 4209
selectable marker ampR ampicillin resistance gene 6687 7544


Author Name Author Type Creation Date
Daniel Silver Academic researcher, vector donor


PMID Title
11511376 Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity

Parent Vector:

Name Comments