Vector: pENTR221

Number of plasmids with this backbone: 244
Name: pENTR221
Description: Gateway type (lambda att-type) recombinational cloning entry (master) vector; kanamycin resistance; recombinational cloning.
Synonyms: pENTR221-XnCDSYn, pDONR221
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 2574
Properties: Gateway, donor (entry), recombinational cloning
Vector Map: pENTR221-map.doc
Vector Sequence: pENTR221-seq.doc
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website http://www.orfeomecollaboration.org/html/vectors.shtml.

Features:

Type Name Description Start Position End Position
recombination site rrnBT2 rrnB T2 transcription termination sequence 275 302
recombination site rrnBT1 rrnB T1 transcription termination sequence 434 477
recombination site attL1 attL1 recombination site 575 675
recombination site attL2 attL2 recombination site 805 706
primer site M13 Rev M13 reverse primer site 846 865
primer site M13 F M13 forward priming site 846 865
selectable marker kanR kanamycin resistance gene 975 1784
bacterial origin pUC ori pUC origin of replication 1905 4

Authors:

Author Name Author Type Creation Date
Invitrogen Commercial Vendor

Publications:

PMID Title

Parent Vector:

Name Comments