Vector: pENTR/D-TOPO

Number of plasmids with this backbone: 11
Description: Gateway type (lambda att-type) recombinational cloning system entry (master) vector for TOPO cloning of insert; kanamycin resistance; recombinational cloning, TOPO cloning.
Synonyms: pENTR-D-TOPO
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 2580
Properties: Gateway, TOPO Cloning, donor (entry), recombinational cloning
Vector Map: pENTR_D-TOPO-map.pdf
Vector Sequence: pENTR_D-TOPO-seq.doc
Comments: Additional information about this vector is available from the commercial website and at the ORFeome website


Type Name Description Start Position End Position
MCS MCS MCS (NotI and AscI sites flank the TOPO cloning site) 0 0
trxn termination sequence rrnB trxn term rrnB T2 transcription termination sequence 268 295
trxn termination sequence rrnB txn term rrnB T1 transcription termination sequence 427 470
primer site M13 F M13 forward priming site 537 552
recombination site attL1 attL1 recombination site 569 668
TOPO recognition site TOPO 1 TOPO recognition site 1 680 684
TOPO recognition site TOPO 2 TOPO recognition site 2 689 693
recombination site attL2 attL2 recombination site 705 804
promoter T7 T7promoter and primer binding site 821 840
primer site M13 Rev M13 reverse primer site 845 861
selectable marker kanR kanamycin resistance gene 974 1783
bacterial origin pUC ori pUC-type origin of replication 1904 2577


Author Name Author Type Creation Date
Invitrogen Commercial Vendor


PMID Title

Parent Vector:

Name Comments