Vector: pKT146
Number of plasmids with this backbone: | 1 |
Name: | pKT146 |
Description: | Yeast fluorescent (tdimer2) vector; amp resistance (HIS5 for yeast); restriction enzyme cloning. |
Synonyms: | pFA6a-link-tdimer2-SpHIS5, pKT0146, pFA6a-link-tdimer2-HIS |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 5416 |
Properties: | fluorescent marker, multiple cloning site, with tag/fusion/marker, yeast expression, yeast shuttle |
Vector Map: | pFA6a-link-tdimer2-SpHIS5.pdf |
Vector Sequence: | pFA6a-link-tdimer2-His_FASTA.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
fluorescent marker | tdimer2 | tdimer2 tandem DsRed coding regions | 10 | 1404 |
selectable marker | HIS5 | S. pombe HIS5 gene (Complements S. cerevisiae HIS3) | 2031 | 2681 |
primer site | reverse primer | reverse primer binding site | 2925 | 2942 |
selectable marker | ampR | ampicillin resistance resistance gene | 4030 | 4887 |
MCS | MCS | MCS (HindIII, BsiWI, PstI, SalI unique) | 5374 | 5391 |
primer site | forward primer | forward primer binding site | 5402 | 3 |
Authors:
Author Name | Author Type | Creation Date |
Kurt Thorn | Academic researcher, vector donor |
Publications:
PMID | Title |
15197731 | Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae |
Parent Vector:
Name | Comments |