Vector: pKT146

Number of plasmids with this backbone: 1
Name: pKT146
Description: Yeast fluorescent (tdimer2) vector; amp resistance (HIS5 for yeast); restriction enzyme cloning.
Synonyms: pFA6a-link-tdimer2-SpHIS5, pKT0146, pFA6a-link-tdimer2-HIS
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 5416
Properties: fluorescent marker, multiple cloning site, with tag/fusion/marker, yeast expression, yeast shuttle
Vector Map: pFA6a-link-tdimer2-SpHIS5.pdf
Vector Sequence: pFA6a-link-tdimer2-His_FASTA.txt
Comments:

Features:

Type Name Description Start Position End Position
fluorescent marker tdimer2 tdimer2 tandem DsRed coding regions 10 1404
selectable marker HIS5 S. pombe HIS5 gene (Complements S. cerevisiae HIS3) 2031 2681
primer site reverse primer reverse primer binding site 2925 2942
selectable marker ampR ampicillin resistance resistance gene 4030 4887
MCS MCS MCS (HindIII, BsiWI, PstI, SalI unique) 5374 5391
primer site forward primer forward primer binding site 5402 3

Authors:

Author Name Author Type Creation Date
Kurt Thorn Academic researcher, vector donor

Publications:

PMID Title
15197731 Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae

Parent Vector:

Name Comments