Vector: pMycoMar

Number of plasmids with this backbone: 1
Name: pMycoMar
Description: Usage notes: 1) pMycoMar can be propagated in standard E. coli cloning strains such as DH5alpha. Strain passage should be minimized to reduce the possibility of plasmid loss due to transposition of the MycoMar transposon out of the plasmid. Please also keep in mind that mariner- containing plasmids can be prone to spontaneous rearrangement. 2) The MycoMar transposon contains an oriR6k origin of replication, which requires the pir protein in trans for replication. The E. coli strain DH5alpha lambda pir is suitable for propagation of oriR6K- containing plasmids and is provided to facilitate recovery of transposon insertion junctions.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 0
Properties: mutagenesis vector, transposon mediated gene disruption
Vector Map: pMycoMar.jpg
Vector Sequence:
Comments: 1) Purified pMycoMar (Rubin, et al., 1999. PMID: 9990078) 2) E. coli strain DH5alpha lambda pir

Features:

Type Name Description Start Position End Position
selectable marker gentR gentamicin resistance gene (inferred) 0 0
selectable marker kanR kanamycin resistance gene (inferred) 0 0
selectable marker tetR tetracycline resistance gene (inferred) 0 0
promoter myco pr mycobacterial promoter (driver for selection of transposon inserts in mycobacteria) 0 0
transposon fragment transposon transposon elements (with selectable marker) 0 0
transposon fragment transpoase transposase source 0 0
bacterial origin ori E. coli origin of replication (inferred) 0 0

Authors:

Author Name Author Type Creation Date
John Mekalanos Academic Researcher, vector PI

Publications:

PMID Title
9990078 In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria

Parent Vector:

Name Comments