Vector: pMEnt

Number of plasmids with this backbone: 1
Name: pMEnt
Description: Mariner transposon fragment-containing vector used to create pFD1; chloramphenicol, kanamycin and tetracycline resistance; restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 0
Properties: mutagenesis vector, transposon mediated gene disruption
Vector Map:
Vector Sequence:
Comments: See also pFD1

Features:

Type Name Description Start Position End Position
selectable marker chlR chloramphenicol resistance gene (inferred) 0 0
selectable marker kanR kanamycin resistance gene 0 0
selectable marker tetR tetracycline resistance gene (inferred) 0 0
bacterial origin ori E. coli origin of replication (inferred) 0 0
transposon fragment transposase Himar1-type transposase 0 0
transposon fragment transposon minitransposon magellan3 with Himar1-type transposon repeats (flanks kanR) 0 0
promoter lac pr lac promoter (drives transposase) 0 0

Authors:

Author Name Author Type Creation Date
John Mekalanos Academic Researcher, vector PI

Publications:

PMID Title
9990078 In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria

Parent Vector:

Name Comments