Vector: pHRST-nCre

Number of plasmids with this backbone: 1
Name: pHRST-nCre
Description: Lentiviral vector that expresses nuclear localization signal (nls)-containing Cre recombinase from internal CMV promoter; ampicillin resistance; restriction enzyme cloning.
Synonyms: pHRST-nlsCre
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 11028
Properties: lentiviral infection, mammalian expression, mammalian transduction, retroviral vector
Vector Map: pHRST-nCre.pdf
Vector Sequence: pHRST-nCre.txt
Comments:

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 871 1530
bacterial origin ColE1 ori ColE1-type origin of replication 1628 2310
promoter SV40 pr SV40 early promoter 2671 2867
viral gene gpt gpt viral gene cds (possibly inactive) 3067 3524
poly-A signal SV40 polyA SV40 late polyA signal sequence 4580 4771
viral LTR LTR viral long terminal repeat (LTR) 4944 5579
promoter U3 U3 promoter 4944 5396
promoter R R promtoer 5397 5495
promoter U5 U5 promoter 5496 5579
packaging signal psi psi viral packaging sequence 5580 6094
response element RRE retroviral response element 6242 6475
intervening sequence cpPu central polypurine track (cpPu, also Trip) 6885 7062
promoter CMV pr CMV promoter (for nls-Cre cds) 7173 7699
Cre coding region nCre nuclear localization sequence-Cre enzyme cds 7793 8845
viral LTR dU3LTR viral long terminal repeat (LTR) 9076 9311
promoter R R promoter 9131 9229
promoter U5 U5 promoter 9230 9313
intervening sequence 3p flank 3 prime flanking region 9312 10649
primer site SP6 SP6 10808 10791

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments