Vector: pHAGE-UBC-MCS-IRES-ZsGreen-W

Number of plasmids with this backbone: 1
Name: pHAGE-UBC-MCS-IRES-ZsGreen-W
Description: Lentiviral vector that expresses insert from internal UBC promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7555
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-UBC-MCS-IZsGreen-W.pdf
Vector Sequence: pHAGE-UBC-MCS-IZsGreen-W.txt
Comments: ZsGreen described as like eGFP but brighter

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 1 636
promoter U3 U3 promoter 1 453
promoter R R promoter 454 552
promoter U5 U5 promoter 553 636
response element RRE retroviral response element 1303 1536
intervening sequence cpPu central polypurine track (cpPu or Trip) 2034 2211
IRES IRES internal ribosome entry site (IRES) 2658 3235
fluorescent marker ZsGreen ZsGreen fluorescent protein cds 3236 3931
response element WPRE woodchuck hepatitis posttranscriptional regulatory element (WPRE) 4044 4535
viral LTR dU3LTR viral long terminal repeat (LTR) 4606 4841
promoter R R promoter 4661 4759
promoter U5 U5 promoter 4760 4843
intervening sequence 3p flank 3 prime flanking region 4842 4912
selectable marker ampR ampicillin resistance gene 5349 6008
bacterial origin ColE1 ori ColE1-type origin of replication 6106 6788
viral origin SV40 ori SV40 origin of replication 7007 7130
repressor binding site LacO LacO sequenc region 7251 7273

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments