Vector: pHAGE-shortEF1a-MCS-IRES-ZsGreen-W

Number of plasmids with this backbone: 1
Name: pHAGE-shortEF1a-MCS-IRES-ZsGreen-W
Description: Lentiviral vector that expresses insert from internal short EF1a promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7386
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-shortEF1a-MCS-IZsGreen-W.pdf
Vector Sequence: pHAGE-shortEF1a-MCS-IZsGreen-W.txt
Comments: Note that there is also a "full EF1a" promoter version in the repository database.

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 1 636
promoter R R promoter 454 552
promoter U5 U5 promoter 553 636
response element RRE retroviral response element 1303 1536
intervening sequence cpPu centra polypurine track (cpPu or Trip) 2034 2211
IRES IRES internal ribosome entry site (IRES) 2489 3066
fluorescent marker ZsGreen ZsGreen fluorescent protein cds 3067 3762
response element WPRE woodchuck hepatitis posttranscriptional regulatory element (WPRE) 3875 4366
viral LTR dU3LTR viral long terminal repeat (LTR) 4437 4671
promoter R R promoter 4492 4590
promoter U5 U5 promoter 4591 4674
intervening sequence 3p flank 3 prime flanking region 4673 4743
selectable marker ampR ampicillin resistance gene 5180 5839
bacterial origin ColE1 ori ColE1-type origin of replication 5937 6619
viral origin SV40 ori SV40 origin of replication 6838 6961
repressor binding site LacO LacO sequence 7082 7104

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments