Name: |
pHAGE-shortEF1a-MCS-IRES-ZsGreen-W |
Description: |
Lentiviral vector that expresses insert from internal short EF1a promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning. |
Synonyms: |
|
Type: |
bacterial plasmid |
Form: |
dsDNA |
Size (bp):: |
7386 |
Properties: |
fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker |
Vector Map: |
pHAGE-shortEF1a-MCS-IZsGreen-W.pdf
|
Vector Sequence: |
pHAGE-shortEF1a-MCS-IZsGreen-W.txt |
Comments: |
Note that there is also a "full EF1a" promoter version in the repository database. |
Type |
Name |
Description |
Start Position |
End Position |
viral LTR |
LTR |
viral long terminal repeat (LTR) |
1 |
636 |
promoter |
R |
R promoter |
454 |
552 |
promoter |
U5 |
U5 promoter |
553 |
636 |
response element |
RRE |
retroviral response element |
1303 |
1536 |
intervening sequence |
cpPu |
centra polypurine track (cpPu or Trip) |
2034 |
2211 |
IRES |
IRES |
internal ribosome entry site (IRES) |
2489 |
3066 |
fluorescent marker |
ZsGreen |
ZsGreen fluorescent protein cds |
3067 |
3762 |
response element |
WPRE |
woodchuck hepatitis posttranscriptional regulatory element (WPRE) |
3875 |
4366 |
viral LTR |
dU3LTR |
viral long terminal repeat (LTR) |
4437 |
4671 |
promoter |
R |
R promoter |
4492 |
4590 |
promoter |
U5 |
U5 promoter |
4591 |
4674 |
intervening sequence |
3p flank |
3 prime flanking region |
4673 |
4743 |
selectable marker |
ampR |
ampicillin resistance gene |
5180 |
5839 |
bacterial origin |
ColE1 ori |
ColE1-type origin of replication |
5937 |
6619 |
viral origin |
SV40 ori |
SV40 origin of replication |
6838 |
6961 |
repressor binding site |
LacO |
LacO sequence |
7082 |
7104 |