Vector: pHAGE-PGK-MCS-IRES-ZsGreen-W

Number of plasmids with this backbone: 1
Name: pHAGE-PGK-MCS-IRES-ZsGreen-W
Description: Lentiviral vector that expresses insert from internal PGK promoter, after MCS for insert is IRES-ZsGreen fluorescent marker, with WPRE; ampicillin resistance; restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7622
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-PGK-MCS-IZsGreen-W.pdf
Vector Sequence: pHAGE-PGK-MCS-IZsGreen-W.txt
Comments: ZsGreen described as like eGFP but brighter

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 1 636
intervening sequence cpPu central polypurine track 2034 2211
IRES IRES internal ribosome entry site (IRES) 2725 3302
intervening sequence 3p flank 3 prime flanking region 2909 2979
fluorescent marker ZsGreen ZsGreen fluorescent protein cds 3303 3998
response element WPRE woodchuck hepatitis posttranscriptional regulatory element (WPRE) 4111 4602
viral LTR dU3LTR viral long terminal repeat (LTR) 4673 4908
selectable marker ampR ampicillin resistance gene 5416 6075
bacterial origin ColE1 ori ColE1-type origin of replication 6173 6855
viral origin SV40 ori SV40 origin of replication 7074 7197
repressor binding site LacO LacO sequence 7318 7340

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments