Vector: pHAGE-CMV-eGFP-W
Number of plasmids with this backbone: | 1 |
Name: | pHAGE-CMV-eGFP-W |
Description: | Lentiviral vector that expresses eGFP fluorescent marker from CMV promoter, with WPRE; ampicillin resistance; restriction enzyme cloning. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 7149 |
Properties: | fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker |
Vector Map: | pHAGE-CMV-eGFP-W.pdf |
Vector Sequence: | pHAGE-CMV-eGFP-W.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
viral LTR | LTR | viral long terminal repeat (LTR) | 1 | 636 |
response element | RRE | RRE response element | 1303 | 1536 |
intervening sequence | cpPu | central polypurine track | 2034 | 2211 |
promoter | CMV pr | CMV promoter (for eGFP expression) | 2218 | 2802 |
fluorescent marker | eGFP | enhanced Green Fluorescent Protein (eGFP) cds | 2812 | 3531 |
response element | WPRE | woodchuck hepatitis posttranscriptional regulatory element (WPRE) | 3638 | 4129 |
viral LTR | dU3LTR | viral long terminal repeat (LTR) | 4200 | 4435 |
intervening sequence | 3p flank | 3 prime flanking region | 4436 | 4506 |
selectable marker | ampR | ampicillin resistance gene | 4745 | 5605 |
bacterial origin | ColE1 | ColE1-type origin of replication | 5700 | 6382 |
viral origin | SV40 ori | SV40 origin of replication | 6601 | 6724 |
Authors:
Author Name | Author Type | Creation Date |
Jeng-Shin Lee | Academic Researcher, vector PI |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |