Vector: pHAGE-CMV-eGFP-W

Number of plasmids with this backbone: 1
Name: pHAGE-CMV-eGFP-W
Description: Lentiviral vector that expresses eGFP fluorescent marker from CMV promoter, with WPRE; ampicillin resistance; restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7149
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-CMV-eGFP-W.pdf
Vector Sequence: pHAGE-CMV-eGFP-W.txt
Comments:

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 1 636
response element RRE RRE response element 1303 1536
intervening sequence cpPu central polypurine track 2034 2211
promoter CMV pr CMV promoter (for eGFP expression) 2218 2802
fluorescent marker eGFP enhanced Green Fluorescent Protein (eGFP) cds 2812 3531
response element WPRE woodchuck hepatitis posttranscriptional regulatory element (WPRE) 3638 4129
viral LTR dU3LTR viral long terminal repeat (LTR) 4200 4435
intervening sequence 3p flank 3 prime flanking region 4436 4506
selectable marker ampR ampicillin resistance gene 4745 5605
bacterial origin ColE1 ColE1-type origin of replication 5700 6382
viral origin SV40 ori SV40 origin of replication 6601 6724

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector PI

Publications:

PMID Title

Parent Vector:

Name Comments