Vector: pKT176

Number of plasmids with this backbone: 1
Name: pKT176
Description: Yeast fluorescent (tdimer2) vector with PTEF promoter; amp resistance (CaURA3 for yeast); restriction enzyme cloning.
Synonyms: pFA6a-link-tdimer2-CaUra3, pKT0176, pFA6a-link-tdimer2-Ura3
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 5578
Properties: fluorescent marker, multiple cloning site, with tag/fusion/marker, yeast expression, yeast shuttle
Vector Map: pFA6a-link-tdimer2-CaUra3.pdf
Vector Sequence: pFA6a-link-tdimer2-Ura3_FASTA.txt
Comments:

Features:

Type Name Description Start Position End Position
fluorescent marker tdimer2 tdimer2 tandem DsRed coding regions 10 1404
promoter PTEF PTEF eukaryotic promoter 1657 2030
selectable marker CaURA3 Candida albicans URA3 gene 2031 2843
selectable marker ampR ampicillin resistance gene 4192 5049
MCS MCS MCS (NotI, HindIII, BsiWI, PstI, SalI unique) 5523 5553
primer site forward primer forward primer binding site 5564 3

Authors:

Author Name Author Type Creation Date
Kurt Thorn Academic researcher, vector donor

Publications:

PMID Title
15197731 Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae

Parent Vector:

Name Comments