Vector: pKT176
Number of plasmids with this backbone: | 1 |
Name: | pKT176 |
Description: | Yeast fluorescent (tdimer2) vector with PTEF promoter; amp resistance (CaURA3 for yeast); restriction enzyme cloning. |
Synonyms: | pFA6a-link-tdimer2-CaUra3, pKT0176, pFA6a-link-tdimer2-Ura3 |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 5578 |
Properties: | fluorescent marker, multiple cloning site, with tag/fusion/marker, yeast expression, yeast shuttle |
Vector Map: | pFA6a-link-tdimer2-CaUra3.pdf |
Vector Sequence: | pFA6a-link-tdimer2-Ura3_FASTA.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
fluorescent marker | tdimer2 | tdimer2 tandem DsRed coding regions | 10 | 1404 |
promoter | PTEF | PTEF eukaryotic promoter | 1657 | 2030 |
selectable marker | CaURA3 | Candida albicans URA3 gene | 2031 | 2843 |
selectable marker | ampR | ampicillin resistance gene | 4192 | 5049 |
MCS | MCS | MCS (NotI, HindIII, BsiWI, PstI, SalI unique) | 5523 | 5553 |
primer site | forward primer | forward primer binding site | 5564 | 3 |
Authors:
Author Name | Author Type | Creation Date |
Kurt Thorn | Academic researcher, vector donor |
Publications:
PMID | Title |
15197731 | Optimized cassettes for fluorescent protein tagging in Saccharomyces cerevisiae |
Parent Vector:
Name | Comments |