Vector: pGP704
Number of plasmids with this backbone: | 1 |
Name: | pGP704 |
Description: | Vector that accepts insert for gene targeting approach in bacteria; ampicillin, streptomycin (tetracycline? See map); restriction enzyme cloning. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 3705 |
Properties: | bacterial expression, gene targeting |
Vector Map: | pGP704.gif |
Vector Sequence: | pGP704_seq.doc |
Comments: |
Type | Name | Description | Start Position | End Position |
gene fragment | tet | tet | 0 | 0 |
selectable marker | ampR | ampicillin resistance gene (from pBR322) | 0 | 0 |
gene fragment | mob | mob/RP4 | 0 | 0 |
bacterial origin | ori | ori/R6K | 0 | 0 |
MCS | MCS | MCS (BglII, SalI, PstI, BamHI, HinDIII, XbaI, KnpI, SphI, EcoRV, SacI, SmaI, EcoRI) | 3633 | 3700 |
Authors:
Author Name | Author Type | Creation Date |
John Mekalanos | Academic Researcher, vector PI |
Publications:
PMID | Title |
2836362 | A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR |
728998 | Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K |
Parent Vector:
Name | Comments |