Vector: pGP704

Number of plasmids with this backbone: 1
Name: pGP704
Description: Vector that accepts insert for gene targeting approach in bacteria; ampicillin, streptomycin (tetracycline? See map); restriction enzyme cloning.
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3705
Properties: bacterial expression, gene targeting
Vector Map: pGP704.gif
Vector Sequence: pGP704_seq.doc


Type Name Description Start Position End Position
gene fragment tet tet 0 0
selectable marker ampR ampicillin resistance gene (from pBR322) 0 0
gene fragment mob mob/RP4 0 0
bacterial origin ori ori/R6K 0 0
MCS MCS MCS (BglII, SalI, PstI, BamHI, HinDIII, XbaI, KnpI, SphI, EcoRV, SacI, SmaI, EcoRI) 3633 3700


Author Name Author Type Creation Date
John Mekalanos Academic Researcher, vector PI


PMID Title
2836362 A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
728998 Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

Parent Vector:

Name Comments