Vector: pGP704

Number of plasmids with this backbone: 1
Name: pGP704
Description: Vector that accepts insert for gene targeting approach in bacteria; ampicillin, streptomycin (tetracycline? See map); restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 3705
Properties: bacterial expression, gene targeting
Vector Map: pGP704.gif
Vector Sequence: pGP704_seq.doc
Comments:

Features:

Type Name Description Start Position End Position
gene fragment tet tet 0 0
selectable marker ampR ampicillin resistance gene (from pBR322) 0 0
gene fragment mob mob/RP4 0 0
bacterial origin ori ori/R6K 0 0
MCS MCS MCS (BglII, SalI, PstI, BamHI, HinDIII, XbaI, KnpI, SphI, EcoRV, SacI, SmaI, EcoRI) 3633 3700

Authors:

Author Name Author Type Creation Date
John Mekalanos Academic Researcher, vector PI

Publications:

PMID Title
2836362 A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR
728998 Trans-complementation-dependent replication of a low molecular weight origin fragment from plasmid R6K

Parent Vector:

Name Comments