Vector: pEnt3
Number of plasmids with this backbone: | 1 |
Name: | pEnt3 |
Description: | Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning. |
Synonyms: | |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 0 |
Properties: | mutagenesis vector, transposon mediated gene disruption |
Vector Map: | pEnt3.pdf |
Vector Sequence: | pEnt3.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
selectable marker | ampR | ampicillin resistance gene (inferred) | 0 | 0 |
selectable marker | Tn-magellan1 | transposon with kanR gene for selection of insertions | 0 | 0 |
transposon fragment | Tn-magellan1 | transposon with kanR gene for selection of insertions | 0 | 0 |
bacterial origin | ori | E. coli origin of replication | 0 | 0 |
Authors:
Author Name | Author Type | Creation Date |
John Mekalanos | Academic Researcher, vector PI |
Publications:
PMID | Title |
10500193 | Hyperactive transposase mutants of the Himar1 mariner transposon |
9671781 | Systematic identification of essential genes by in vitro mariner mutagenesis |
Parent Vector:
Name | Comments |