Vector: pEnt3

Number of plasmids with this backbone: 1
Name: pEnt3
Description: Vector for transposon-mediated mutagenesis in bacteria, with Tn-magellan1 element; ampicillin and kanamycin resistance (kanamycin for selection of transposon insertions); restriction enzyme cloning.
Synonyms:
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 0
Properties: mutagenesis vector, transposon mediated gene disruption
Vector Map: pEnt3.pdf
Vector Sequence: pEnt3.txt
Comments:

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene (inferred) 0 0
selectable marker Tn-magellan1 transposon with kanR gene for selection of insertions 0 0
transposon fragment Tn-magellan1 transposon with kanR gene for selection of insertions 0 0
bacterial origin ori E. coli origin of replication 0 0

Authors:

Author Name Author Type Creation Date
John Mekalanos Academic Researcher, vector PI

Publications:

PMID Title
10500193 Hyperactive transposase mutants of the Himar1 mariner transposon
9671781 Systematic identification of essential genes by in vitro mariner mutagenesis

Parent Vector:

Name Comments