Vector: pHRST-IRES-eGFP

Number of plasmids with this backbone: 1
Name: pHRST-IRES-eGFP
Description: Lentiviral vector with internal CMV promoter, has IRES-eGFP (IRES-fluorescent tag) to mark transduced cells; amp resistance; restriction enzyme cloning.
Synonyms: pHRST-IRESeGFP, pHRSTIRESeGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 11119
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHRST-IRES-GFP.pdf
Vector Sequence: pHRST-IRES-GFP.txt
Comments:

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 0 0
viral response element RRE rev response element 0 0
intervening sequence cpPu central polypurine track 0 0
promoter CMV pr CMV promoter for mammalian expression 0 0
MCS MCS MCS (NotI, BamHI between CMVp and IRES) 0 0
IRES IRES internal ribosome entry site (IRES) 0 0
poly-A signal SV40 late polyA SV40 late polyA signal sequence 0 0
viral LTR dU3LTR viral long terminal repeat (LTR) 0 0
primer site SP6 SP6 primer site 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ColE1 ColE1-type origin of replication 0 0
promoter SV40 early pr SV40 early promoter 0 0
fluorescent marker eGFP eGFP fluorescent coding sequence 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments