Vector: pHRST-IRES-eGFP
Number of plasmids with this backbone: | 1 |
Name: | pHRST-IRES-eGFP |
Description: | Lentiviral vector with internal CMV promoter, has IRES-eGFP (IRES-fluorescent tag) to mark transduced cells; amp resistance; restriction enzyme cloning. |
Synonyms: | pHRST-IRESeGFP, pHRSTIRESeGFP |
Type: | bacterial plasmid |
Form: | dsDNA |
Size (bp):: | 11119 |
Properties: | fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker |
Vector Map: | pHRST-IRES-GFP.pdf |
Vector Sequence: | pHRST-IRES-GFP.txt |
Comments: |
Type | Name | Description | Start Position | End Position |
viral LTR | LTR | viral long terminal repeat (LTR) | 0 | 0 |
viral response element | RRE | rev response element | 0 | 0 |
intervening sequence | cpPu | central polypurine track | 0 | 0 |
promoter | CMV pr | CMV promoter for mammalian expression | 0 | 0 |
MCS | MCS | MCS (NotI, BamHI between CMVp and IRES) | 0 | 0 |
IRES | IRES | internal ribosome entry site (IRES) | 0 | 0 |
poly-A signal | SV40 late polyA | SV40 late polyA signal sequence | 0 | 0 |
viral LTR | dU3LTR | viral long terminal repeat (LTR) | 0 | 0 |
primer site | SP6 | SP6 primer site | 0 | 0 |
selectable marker | ampR | ampicillin resistance gene | 0 | 0 |
bacterial origin | ColE1 | ColE1-type origin of replication | 0 | 0 |
promoter | SV40 early pr | SV40 early promoter | 0 | 0 |
fluorescent marker | eGFP | eGFP fluorescent coding sequence | 0 | 0 |
Authors:
Author Name | Author Type | Creation Date |
Jeng-Shin Lee | Academic Researcher, vector donor and PI |
Publications:
PMID | Title |
Parent Vector:
Name | Comments |