Vector: pHRST-eGFP

Number of plasmids with this backbone: 1
Name: pHRST-eGFP
Description: Lentiviral vector with internal CMV promoter that drives eGFP fluorescent tag; amp resistance; restriction enzyme cloning.
Synonyms: pHRSTeGFP
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 10641
Properties: fluorescent marker, lentiviral infection, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHRST-eGFP.pdf
Vector Sequence: pHRST-eGFP.txt
Comments:

Features:

Type Name Description Start Position End Position
selectable marker ampR ampicillin resistance gene 0 0
primer site SP6 SP6 primer site 0 0
promoter SV40 p/e SV40 early promoter for bacterial expression (drives gpt) 0 0
viral gene gpt gpt (likely inactive) 0 0
poly-A signal Sv40 late polyA SV40 late polyA signal sequence 0 0
promoter U3 U3 promoter (part of viral LTR) 0 0
promoter R R promoter (part of viral LTR) 0 0
promoter U5 U5 promoter (part of viral LTR) 0 0
intervening sequence psi psi 0 0
viral response element rre rev response element (RRE) 0 0
viral gene Trp Trp gene 0 0
promoter CMVp CMV promoter for mammalian expression 0 0
fluorescent marker eGFP eGFP fluorescent coding sequence 0 0
promoter dU3 dU3 promoter (part of viral LTR) 0 0
promoter R R promoter (part of viral LTR) 0 0
promoter U5 U5 promoter (part of viral LTR) 0 0
intervening sequence 3' flanking 3' flanking regin 0 0
bacterial origin ColE1 ori ColE1-type origin of replication 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments