Vector: pHDM-G

Number of plasmids with this backbone: 1
Name: pHDM-G
Description: Helper plasmid for murine leukemia virus (MLV) or lentiviral vectors, CMV promoter drives VSV-G envelope protein, contains human beta globin intron and polyA; amp resistance; not a cloning vector.
Synonyms: pHDMG
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 6237
Properties: MLV helper, lentiviral helper, lentiviral infection, mammalian expression, mammalian transduction
Vector Map: pHDM-G.pdf
Vector Sequence: pHDM-G.txt
Comments: For use as helper with lentiviral vector, separate source of rev (e.g. pRC/CMV-rev1b) is needed.

Features:

Type Name Description Start Position End Position
promoter CMVp CMV promoter for mammalian expression 0 0
viral gene VSV-G VSV-G envelope protein coding sequence 0 0
MCS MCS MCS (BamHI, ClaI, SalI, XhoI, BglII) 0 0
poly-A signal hbg pA human beta globin polyA site 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ori bacterial origin of replication (inferred) 0 0
viral gene VSV-G viral VSV-G gene 0 0
MCS MCS MCS (BamHI, ClaI, SalI, XhoI, BglII) 0 0
poly-A signal hbg pA human beta globin polyA signal sequence 0 0
selectable marker ampR ampicillin resistance gene 0 0
promoter CMV pr CMV promoter for mammalian expression 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments