Vector: pHAGE-CMV-MCS-IRES-ZsGreen

Number of plasmids with this backbone: 1
Name: pHAGE-CMV-MCS-IRES-ZsGreen
Description: Lentiviral vector with internal CMV promoter, has IRES-ZsGreen (IRES-fluorescent tag); amp resistance; restriction enzyme cloning.
Synonyms: pHAGE-CMV-IRES-ZsGreen, pHAGE-CMV-MCS-IRES-ZsGreen-W
Type: bacterial plasmid
Form: dsDNA
Size (bp):: 7742
Properties: fluorescent marker, mammalian expression, mammalian transduction, multiple cloning site, retroviral vector, with tag/fusion/marker
Vector Map: pHAGE-CMV-MCS-IZsGreen.pdf
Vector Sequence: pHAGE-CMV-MCS-IZsGreen.txt
Comments: ZsGreen reported to be like GFP but brighter (statement not evaluated by the DNA Resource Core).

Features:

Type Name Description Start Position End Position
viral LTR LTR viral long terminal repeat (LTR) 0 0
viral response element RRE rev response element 0 0
intervening sequence ccPu central polypurine track 0 0
promoter CMV pr CMV promoter for mammalian expression 0 0
IRES IRES internal ribosome entry site (IRES) 0 0
fluorescent marker ZsGreen ZsGreen fluorescent coding sequence (reportedly like GFP but brighter) 0 0
bacterial operon LacO LacO fragment 0 0
viral LTR dU3 LTR viral long terminal repeat (LTR) 0 0
intervening sequence 3' flank 3' flanking fragment 0 0
selectable marker ampR ampicillin resistance gene 0 0
bacterial origin ColE1 ori ColE1 origin of replication 0 0
viral origin SV40 ori SV40 origin (and promoter) 0 0
viral response element WPRE WPRE response element 0 0

Authors:

Author Name Author Type Creation Date
Jeng-Shin Lee Academic Researcher, vector donor and PI

Publications:

PMID Title

Parent Vector:

Name Comments